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Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode
A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were character...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372010/ https://www.ncbi.nlm.nih.gov/pubmed/25834434 http://dx.doi.org/10.2147/IJN.S76200 |
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author | Zhong, Guangxian Lan, Ruilong Zhang, Wenxin Fu, Feihuan Sun, Yiming Peng, Huaping Chen, Tianbin Cai, Yishan Liu, Ailin Lin, Jianhua Lin, Xinhua |
author_facet | Zhong, Guangxian Lan, Ruilong Zhang, Wenxin Fu, Feihuan Sun, Yiming Peng, Huaping Chen, Tianbin Cai, Yishan Liu, Ailin Lin, Jianhua Lin, Xinhua |
author_sort | Zhong, Guangxian |
collection | PubMed |
description | A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis. |
format | Online Article Text |
id | pubmed-4372010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43720102015-04-01 Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode Zhong, Guangxian Lan, Ruilong Zhang, Wenxin Fu, Feihuan Sun, Yiming Peng, Huaping Chen, Tianbin Cai, Yishan Liu, Ailin Lin, Jianhua Lin, Xinhua Int J Nanomedicine Original Research A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis. Dove Medical Press 2015-03-19 /pmc/articles/PMC4372010/ /pubmed/25834434 http://dx.doi.org/10.2147/IJN.S76200 Text en © 2015 Zhong et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Zhong, Guangxian Lan, Ruilong Zhang, Wenxin Fu, Feihuan Sun, Yiming Peng, Huaping Chen, Tianbin Cai, Yishan Liu, Ailin Lin, Jianhua Lin, Xinhua Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
title | Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
title_full | Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
title_fullStr | Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
title_full_unstemmed | Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
title_short | Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
title_sort | sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372010/ https://www.ncbi.nlm.nih.gov/pubmed/25834434 http://dx.doi.org/10.2147/IJN.S76200 |
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