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A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exoge...

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Detalles Bibliográficos
Autores principales: Rivera-Fuentes, Pablo, Wrobel, Alexandra T., Zastrow, Melissa L., Khan, Mustafa, Georgiou, John, Luyben, Thomas T., Roder, John C., Okamoto, Kenichi, Lippard, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372157/
https://www.ncbi.nlm.nih.gov/pubmed/25815162
http://dx.doi.org/10.1039/c4sc03388d
Descripción
Sumario:Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn(2+) in lysosomes of HeLa cells, endogenous Zn(2+) in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy.