Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism

The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characteriz...

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Detalles Bibliográficos
Autores principales: Stavrinides, Anna, Tatsis, Evangelos C., Foureau, Emilien, Caputi, Lorenzo, Kellner, Franziska, Courdavault, Vincent, O’Connor, Sarah E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372254/
https://www.ncbi.nlm.nih.gov/pubmed/25772467
http://dx.doi.org/10.1016/j.chembiol.2015.02.006
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author Stavrinides, Anna
Tatsis, Evangelos C.
Foureau, Emilien
Caputi, Lorenzo
Kellner, Franziska
Courdavault, Vincent
O’Connor, Sarah E.
author_facet Stavrinides, Anna
Tatsis, Evangelos C.
Foureau, Emilien
Caputi, Lorenzo
Kellner, Franziska
Courdavault, Vincent
O’Connor, Sarah E.
author_sort Stavrinides, Anna
collection PubMed
description The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway.
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spelling pubmed-43722542015-04-01 Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism Stavrinides, Anna Tatsis, Evangelos C. Foureau, Emilien Caputi, Lorenzo Kellner, Franziska Courdavault, Vincent O’Connor, Sarah E. Chem Biol Brief Communication The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway. Elsevier 2015-03-19 /pmc/articles/PMC4372254/ /pubmed/25772467 http://dx.doi.org/10.1016/j.chembiol.2015.02.006 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Communication
Stavrinides, Anna
Tatsis, Evangelos C.
Foureau, Emilien
Caputi, Lorenzo
Kellner, Franziska
Courdavault, Vincent
O’Connor, Sarah E.
Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism
title Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism
title_full Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism
title_fullStr Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism
title_full_unstemmed Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism
title_short Unlocking the Diversity of Alkaloids in Catharanthus roseus: Nuclear Localization Suggests Metabolic Channeling in Secondary Metabolism
title_sort unlocking the diversity of alkaloids in catharanthus roseus: nuclear localization suggests metabolic channeling in secondary metabolism
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372254/
https://www.ncbi.nlm.nih.gov/pubmed/25772467
http://dx.doi.org/10.1016/j.chembiol.2015.02.006
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