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Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates

There is evidence to suggest that the yaws bacterium (Treponema pallidum ssp. pertenue) may exist in non-human primate populations residing in regions where yaws is endemic in humans. Especially in light of the fact that the World Health Organizaiton (WHO) recently launched its second yaws eradicati...

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Autores principales: Knauf, Sascha, Dahlmann, Franziska, Batamuzi, Emmanuel K., Frischmann, Sieghard, Liu, Hsi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372418/
https://www.ncbi.nlm.nih.gov/pubmed/25803295
http://dx.doi.org/10.1371/journal.pntd.0003637
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author Knauf, Sascha
Dahlmann, Franziska
Batamuzi, Emmanuel K.
Frischmann, Sieghard
Liu, Hsi
author_facet Knauf, Sascha
Dahlmann, Franziska
Batamuzi, Emmanuel K.
Frischmann, Sieghard
Liu, Hsi
author_sort Knauf, Sascha
collection PubMed
description There is evidence to suggest that the yaws bacterium (Treponema pallidum ssp. pertenue) may exist in non-human primate populations residing in regions where yaws is endemic in humans. Especially in light of the fact that the World Health Organizaiton (WHO) recently launched its second yaws eradication campaign, there is a considerable need for reliable tools to identify treponemal infection in our closest relatives, African monkeys and great apes. It was hypothesized that commercially available serological tests detect simian anti-T. pallidum antibody in serum samples of baboons, with comparable sensitivity and specificity to their results on human sera. Test performances of five different treponemal tests (TTs) and two non-treponemal tests (NTTs) were evaluated using serum samples of 57 naturally T. pallidum-infected olive baboons (Papio anubis) from Lake Manyara National Park in Tanzania. The T. pallidum particle agglutination assay (TP-PA) was used as a gold standard for comparison. In addition, the overall infection status of the animals was used to further validate test performances. For most accurate results, only samples that originated from baboons of known infection status, as verified in a previous study by clinical inspection, PCR and immunohistochemistry, were included. All tests, TTs and NTTs, used in this study were able to reliably detect antibodies against T. pallidum in serum samples of infected baboons. The sensitivity of TTs ranged from 97.7-100%, while specificity was between 88.0-100.0%. The two NTTs detected anti-lipoidal antibodies in serum samples of infected baboons with a sensitivity of 83.3% whereas specificity was 100%. For screening purposes, the TT Espline TP provided the highest sensitivity and specificity and at the same time provided the most suitable format for use in the field. The enzyme immune assay Mastblot TP (IgG), however, could be considered as a confirmatory test.
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spelling pubmed-43724182015-04-04 Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates Knauf, Sascha Dahlmann, Franziska Batamuzi, Emmanuel K. Frischmann, Sieghard Liu, Hsi PLoS Negl Trop Dis Research Article There is evidence to suggest that the yaws bacterium (Treponema pallidum ssp. pertenue) may exist in non-human primate populations residing in regions where yaws is endemic in humans. Especially in light of the fact that the World Health Organizaiton (WHO) recently launched its second yaws eradication campaign, there is a considerable need for reliable tools to identify treponemal infection in our closest relatives, African monkeys and great apes. It was hypothesized that commercially available serological tests detect simian anti-T. pallidum antibody in serum samples of baboons, with comparable sensitivity and specificity to their results on human sera. Test performances of five different treponemal tests (TTs) and two non-treponemal tests (NTTs) were evaluated using serum samples of 57 naturally T. pallidum-infected olive baboons (Papio anubis) from Lake Manyara National Park in Tanzania. The T. pallidum particle agglutination assay (TP-PA) was used as a gold standard for comparison. In addition, the overall infection status of the animals was used to further validate test performances. For most accurate results, only samples that originated from baboons of known infection status, as verified in a previous study by clinical inspection, PCR and immunohistochemistry, were included. All tests, TTs and NTTs, used in this study were able to reliably detect antibodies against T. pallidum in serum samples of infected baboons. The sensitivity of TTs ranged from 97.7-100%, while specificity was between 88.0-100.0%. The two NTTs detected anti-lipoidal antibodies in serum samples of infected baboons with a sensitivity of 83.3% whereas specificity was 100%. For screening purposes, the TT Espline TP provided the highest sensitivity and specificity and at the same time provided the most suitable format for use in the field. The enzyme immune assay Mastblot TP (IgG), however, could be considered as a confirmatory test. Public Library of Science 2015-03-24 /pmc/articles/PMC4372418/ /pubmed/25803295 http://dx.doi.org/10.1371/journal.pntd.0003637 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Knauf, Sascha
Dahlmann, Franziska
Batamuzi, Emmanuel K.
Frischmann, Sieghard
Liu, Hsi
Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates
title Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates
title_full Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates
title_fullStr Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates
title_full_unstemmed Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates
title_short Validation of Serological Tests for the Detection of Antibodies Against Treponema pallidum in Nonhuman Primates
title_sort validation of serological tests for the detection of antibodies against treponema pallidum in nonhuman primates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372418/
https://www.ncbi.nlm.nih.gov/pubmed/25803295
http://dx.doi.org/10.1371/journal.pntd.0003637
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