Cargando…
Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells
Despite the high prevalence of chronic gastritis caused by H. pylori, the gastric mucosa has received little investigative attention as a unique immune environment. Here, we analyzed whether retinoic acid (RA), an important homeostatic factor in the small intestinal mucosa, also contributes to gastr...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372513/ https://www.ncbi.nlm.nih.gov/pubmed/25249167 http://dx.doi.org/10.1038/mi.2014.86 |
_version_ | 1782363190766600192 |
---|---|
author | Bimczok, Diane Kao, John Y. Zhang, Min Cochrun, Steven Mannon, Peter Peter, Shajan Wilcox, Charles M. Mönkemüller, Klaus E. Harris, Paul R. Grams, Jayleen M. Stahl, Richard D. Smith, Phillip D. Smythies, Lesley E. |
author_facet | Bimczok, Diane Kao, John Y. Zhang, Min Cochrun, Steven Mannon, Peter Peter, Shajan Wilcox, Charles M. Mönkemüller, Klaus E. Harris, Paul R. Grams, Jayleen M. Stahl, Richard D. Smith, Phillip D. Smythies, Lesley E. |
author_sort | Bimczok, Diane |
collection | PubMed |
description | Despite the high prevalence of chronic gastritis caused by H. pylori, the gastric mucosa has received little investigative attention as a unique immune environment. Here, we analyzed whether retinoic acid (RA), an important homeostatic factor in the small intestinal mucosa, also contributes to gastric immune regulation. We report that human gastric tissue contains high levels of the RA precursor molecule, retinol, and that gastric epithelial cells express both RA biosynthesis genes and RA response genes, indicative of active RA biosynthesis. Moreover, primary gastric epithelial cells cultured in the presence of retinol synthesized RA in vitro and induced RA biosynthesis in co-cultured monocytes through an RA-dependent mechanism, suggesting that gastric epithelial cells may also confer the ability to generate RA on gastric DCs. Indeed, DCs purified from gastric mucosa had similar levels of aldehyde dehydrogenase activity and RA biosynthesis gene expression as small intestinal DCs, although gastric DCs lacked CD103. In H. pylori-infected gastric mucosa, gastric RA biosynthesis gene expression was severely disrupted, which may lead to reduced RA signaling and thus contribute to disease progression. Collectively, our results support a critical role for RA in human gastric immune regulation. |
format | Online Article Text |
id | pubmed-4372513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-43725132015-11-01 Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells Bimczok, Diane Kao, John Y. Zhang, Min Cochrun, Steven Mannon, Peter Peter, Shajan Wilcox, Charles M. Mönkemüller, Klaus E. Harris, Paul R. Grams, Jayleen M. Stahl, Richard D. Smith, Phillip D. Smythies, Lesley E. Mucosal Immunol Article Despite the high prevalence of chronic gastritis caused by H. pylori, the gastric mucosa has received little investigative attention as a unique immune environment. Here, we analyzed whether retinoic acid (RA), an important homeostatic factor in the small intestinal mucosa, also contributes to gastric immune regulation. We report that human gastric tissue contains high levels of the RA precursor molecule, retinol, and that gastric epithelial cells express both RA biosynthesis genes and RA response genes, indicative of active RA biosynthesis. Moreover, primary gastric epithelial cells cultured in the presence of retinol synthesized RA in vitro and induced RA biosynthesis in co-cultured monocytes through an RA-dependent mechanism, suggesting that gastric epithelial cells may also confer the ability to generate RA on gastric DCs. Indeed, DCs purified from gastric mucosa had similar levels of aldehyde dehydrogenase activity and RA biosynthesis gene expression as small intestinal DCs, although gastric DCs lacked CD103. In H. pylori-infected gastric mucosa, gastric RA biosynthesis gene expression was severely disrupted, which may lead to reduced RA signaling and thus contribute to disease progression. Collectively, our results support a critical role for RA in human gastric immune regulation. 2014-09-24 2015-05 /pmc/articles/PMC4372513/ /pubmed/25249167 http://dx.doi.org/10.1038/mi.2014.86 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Bimczok, Diane Kao, John Y. Zhang, Min Cochrun, Steven Mannon, Peter Peter, Shajan Wilcox, Charles M. Mönkemüller, Klaus E. Harris, Paul R. Grams, Jayleen M. Stahl, Richard D. Smith, Phillip D. Smythies, Lesley E. Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells |
title | Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells |
title_full | Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells |
title_fullStr | Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells |
title_full_unstemmed | Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells |
title_short | Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells |
title_sort | human gastric epithelial cells contribute to gastric immune regulation by providing retinoic acid to dendritic cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372513/ https://www.ncbi.nlm.nih.gov/pubmed/25249167 http://dx.doi.org/10.1038/mi.2014.86 |
work_keys_str_mv | AT bimczokdiane humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT kaojohny humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT zhangmin humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT cochrunsteven humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT mannonpeter humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT petershajan humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT wilcoxcharlesm humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT monkemullerklause humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT harrispaulr humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT gramsjayleenm humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT stahlrichardd humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT smithphillipd humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells AT smythieslesleye humangastricepithelialcellscontributetogastricimmuneregulationbyprovidingretinoicacidtodendriticcells |