The impact of diclofenac and ibuprofen on biofilm formation on the surface of polypropylene mesh

PURPOSE: The difficulties related to treatment of deep surgical site infection involve formation of biofilm on the surface of synthetic material. It is considered that in treatment of infections involving formation of biofilm, concomitant therapy shall be applied covering anti-inflammatory drugs. The purpose of the work was to assess the impact of diclofenac and ibuprofen on bacterial biofilm formation on the surface of monofilament polypropylene mesh. MATERIALS AND METHODS: The study involved 70 strains of Staphylococcus aureus and 70 strains of Escherichia coli isolated from different patients and those which differ with chromosomal DNA pattern within the species. The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM). RESULTS: In the qualitative assessment, after incubation in the medium containing NSAIDs statistically significant growth of S. aureus strain amount and E. coli which poorly make up biofilm was stated. Quantitative examination indicated characteristic decrease of the number of colony forming units in 1 ml of the suspension isolated from bacterial biofilm formed as a result of incubation of isolates in the medium with the addition of examined NSAIDs in comparison to biofilm from control regimen. In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface. CONCLUSIONS: Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.