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Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts
Introduction: Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. In a recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. The aim of the present study wa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Center for Endodontic Research
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372780/ https://www.ncbi.nlm.nih.gov/pubmed/25834590 |
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author | Parirokh, Masoud Forghani, Farshid Reza Paseban, Hamzeh Asgary, Saeed Askarifard, Sara Esmaeeli Mahani, Saeed |
author_facet | Parirokh, Masoud Forghani, Farshid Reza Paseban, Hamzeh Asgary, Saeed Askarifard, Sara Esmaeeli Mahani, Saeed |
author_sort | Parirokh, Masoud |
collection | PubMed |
description | Introduction: Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. In a recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. The aim of the present study was to compare the cytotoxicity of Duraflur varnish with two popular commonly used root canal sealers (AH-Plus and AH-26) on human gingival fibroblasts (HGF). Methods and Materials: The HGFs were incubated with different concentrations (1/2, 1/4, and 1/8) of AH-plus, AH-26, and Duraflur varnish for 24 h. The percentage of cell viability was assessed with methyl-thiazol-tetrazolium (MTT) assay. The data was analyzed using the one-way ANOVA followed by Student-Newman-Keuls test. The level of significance was set at 0.001. Results: MTT assay showed that higher concentrations of the tested materials resulted in lower viability of HGFs. AH-Plus showed significantly greater cell viability compared to AH-26 at all dilutions (P<0.001); however, no significant difference was found between Duraflur and AH-Plus in terms of cell viability at 1/8 dilution (P>0.001). Duraflur showed significantly higher cell viability compared to AH-26 except at 1/2 dilution (P<0.001). Conclusion: Although Duraflur varnish had better biocompatibility compared to AH-26, it should still be evaluated with further biocompatibility tests such as intraosseous and subcutaneous implantation. |
format | Online Article Text |
id | pubmed-4372780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Iranian Center for Endodontic Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-43727802015-04-01 Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts Parirokh, Masoud Forghani, Farshid Reza Paseban, Hamzeh Asgary, Saeed Askarifard, Sara Esmaeeli Mahani, Saeed Iran Endod J Original Article Introduction: Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. In a recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. The aim of the present study was to compare the cytotoxicity of Duraflur varnish with two popular commonly used root canal sealers (AH-Plus and AH-26) on human gingival fibroblasts (HGF). Methods and Materials: The HGFs were incubated with different concentrations (1/2, 1/4, and 1/8) of AH-plus, AH-26, and Duraflur varnish for 24 h. The percentage of cell viability was assessed with methyl-thiazol-tetrazolium (MTT) assay. The data was analyzed using the one-way ANOVA followed by Student-Newman-Keuls test. The level of significance was set at 0.001. Results: MTT assay showed that higher concentrations of the tested materials resulted in lower viability of HGFs. AH-Plus showed significantly greater cell viability compared to AH-26 at all dilutions (P<0.001); however, no significant difference was found between Duraflur and AH-Plus in terms of cell viability at 1/8 dilution (P>0.001). Duraflur showed significantly higher cell viability compared to AH-26 except at 1/2 dilution (P<0.001). Conclusion: Although Duraflur varnish had better biocompatibility compared to AH-26, it should still be evaluated with further biocompatibility tests such as intraosseous and subcutaneous implantation. Iranian Center for Endodontic Research 2015 2015-03-18 /pmc/articles/PMC4372780/ /pubmed/25834590 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Parirokh, Masoud Forghani, Farshid Reza Paseban, Hamzeh Asgary, Saeed Askarifard, Sara Esmaeeli Mahani, Saeed Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts |
title | Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts |
title_full | Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts |
title_fullStr | Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts |
title_full_unstemmed | Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts |
title_short | Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts |
title_sort | cytotoxicity of two resin-based sealers and a fluoride varnish on human gingival fibroblasts |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372780/ https://www.ncbi.nlm.nih.gov/pubmed/25834590 |
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