Cargando…

A Modular Analysis of the Auxin Signalling Network

Auxin is essential for plant development from embryogenesis onwards. Auxin acts in large part through regulation of transcription. The proteins acting in the signalling pathway regulating transcription downstream of auxin have been identified as well as the interactions between these proteins, thus...

Descripción completa

Detalles Bibliográficos
Autores principales: Farcot, Etienne, Lavedrine, Cyril, Vernoux, Teva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4373724/
https://www.ncbi.nlm.nih.gov/pubmed/25807071
http://dx.doi.org/10.1371/journal.pone.0122231
_version_ 1782363371372281856
author Farcot, Etienne
Lavedrine, Cyril
Vernoux, Teva
author_facet Farcot, Etienne
Lavedrine, Cyril
Vernoux, Teva
author_sort Farcot, Etienne
collection PubMed
description Auxin is essential for plant development from embryogenesis onwards. Auxin acts in large part through regulation of transcription. The proteins acting in the signalling pathway regulating transcription downstream of auxin have been identified as well as the interactions between these proteins, thus identifying the topology of this network implicating 54 Auxin Response Factor (ARF) and Aux/IAA (IAA) transcriptional regulators. Here, we study the auxin signalling pathway by means of mathematical modeling at the single cell level. We proceed analytically, by considering the role played by five functional modules into which the auxin pathway can be decomposed: the sequestration of ARF by IAA, the transcriptional repression by IAA, the dimer formation amongst ARFs and IAAs, the feedback loop on IAA and the auxin induced degradation of IAA proteins. Focusing on these modules allows assessing their function within the dynamics of auxin signalling. One key outcome of this analysis is that there are both specific and overlapping functions between all the major modules of the signaling pathway. This suggests a combinatorial function of the modules in optimizing the speed and amplitude of auxin-induced transcription. Our work allows identifying potential functions for homo- and hetero-dimerization of transcriptional regulators, with ARF:IAA, IAA:IAA and ARF:ARF dimerization respectively controlling the amplitude, speed and sensitivity of the response and a synergistic effect of the interaction of IAA with transcriptional repressors on these characteristics of the signaling pathway. Finally, we also suggest experiments which might allow disentangling the structure of the auxin signaling pathway and analysing further its function in plants.
format Online
Article
Text
id pubmed-4373724
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-43737242015-03-27 A Modular Analysis of the Auxin Signalling Network Farcot, Etienne Lavedrine, Cyril Vernoux, Teva PLoS One Research Article Auxin is essential for plant development from embryogenesis onwards. Auxin acts in large part through regulation of transcription. The proteins acting in the signalling pathway regulating transcription downstream of auxin have been identified as well as the interactions between these proteins, thus identifying the topology of this network implicating 54 Auxin Response Factor (ARF) and Aux/IAA (IAA) transcriptional regulators. Here, we study the auxin signalling pathway by means of mathematical modeling at the single cell level. We proceed analytically, by considering the role played by five functional modules into which the auxin pathway can be decomposed: the sequestration of ARF by IAA, the transcriptional repression by IAA, the dimer formation amongst ARFs and IAAs, the feedback loop on IAA and the auxin induced degradation of IAA proteins. Focusing on these modules allows assessing their function within the dynamics of auxin signalling. One key outcome of this analysis is that there are both specific and overlapping functions between all the major modules of the signaling pathway. This suggests a combinatorial function of the modules in optimizing the speed and amplitude of auxin-induced transcription. Our work allows identifying potential functions for homo- and hetero-dimerization of transcriptional regulators, with ARF:IAA, IAA:IAA and ARF:ARF dimerization respectively controlling the amplitude, speed and sensitivity of the response and a synergistic effect of the interaction of IAA with transcriptional repressors on these characteristics of the signaling pathway. Finally, we also suggest experiments which might allow disentangling the structure of the auxin signaling pathway and analysing further its function in plants. Public Library of Science 2015-03-25 /pmc/articles/PMC4373724/ /pubmed/25807071 http://dx.doi.org/10.1371/journal.pone.0122231 Text en © 2015 Farcot et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Farcot, Etienne
Lavedrine, Cyril
Vernoux, Teva
A Modular Analysis of the Auxin Signalling Network
title A Modular Analysis of the Auxin Signalling Network
title_full A Modular Analysis of the Auxin Signalling Network
title_fullStr A Modular Analysis of the Auxin Signalling Network
title_full_unstemmed A Modular Analysis of the Auxin Signalling Network
title_short A Modular Analysis of the Auxin Signalling Network
title_sort modular analysis of the auxin signalling network
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4373724/
https://www.ncbi.nlm.nih.gov/pubmed/25807071
http://dx.doi.org/10.1371/journal.pone.0122231
work_keys_str_mv AT farcotetienne amodularanalysisoftheauxinsignallingnetwork
AT lavedrinecyril amodularanalysisoftheauxinsignallingnetwork
AT vernouxteva amodularanalysisoftheauxinsignallingnetwork
AT farcotetienne modularanalysisoftheauxinsignallingnetwork
AT lavedrinecyril modularanalysisoftheauxinsignallingnetwork
AT vernouxteva modularanalysisoftheauxinsignallingnetwork