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A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning
Insect Gustatory and Odorant Receptors (GRs and ORs) form a superfamily of novel transmembrane proteins, which are expressed in chemosensory neurons that detect environmental stimuli. Here we identify homologues of GRs (Gustatory receptor-like (Grl) genes) in genomes across Protostomia, Deuterostomi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374167/ https://www.ncbi.nlm.nih.gov/pubmed/25692633 http://dx.doi.org/10.1038/ncomms7243 |
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author | Saina, Michael Busengdal, Henriette Sinigaglia, Chiara Petrone, Libero Oliveri, Paola Rentzsch, Fabian Benton, Richard |
author_facet | Saina, Michael Busengdal, Henriette Sinigaglia, Chiara Petrone, Libero Oliveri, Paola Rentzsch, Fabian Benton, Richard |
author_sort | Saina, Michael |
collection | PubMed |
description | Insect Gustatory and Odorant Receptors (GRs and ORs) form a superfamily of novel transmembrane proteins, which are expressed in chemosensory neurons that detect environmental stimuli. Here we identify homologues of GRs (Gustatory receptor-like (Grl) genes) in genomes across Protostomia, Deuterostomia and non-Bilateria. Surprisingly, two Grls in the cnidarian Nematostella vectensis, NvecGrl1 and NvecGrl2, are expressed early in development, in the blastula and gastrula, but not at later stages when a putative chemosensory organ forms. NvecGrl1 transcripts are detected around the arboral pole, considered the equivalent to the head-forming region of Bilateria. Morpholino-mediated knockdown of NvecGrl1 causes developmental patterning defects of this region, leading to animals lacking the apical sensory organ. A deuterostome Grl from the sea urchin Strongylocentrotus purpuratus displays similar patterns of developmental expression. These results reveal an early evolutionary origin of the insect chemosensory receptor family, and raise the possibility that their ancestral role was in embryonic development. |
format | Online Article Text |
id | pubmed-4374167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-43741672015-08-18 A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning Saina, Michael Busengdal, Henriette Sinigaglia, Chiara Petrone, Libero Oliveri, Paola Rentzsch, Fabian Benton, Richard Nat Commun Article Insect Gustatory and Odorant Receptors (GRs and ORs) form a superfamily of novel transmembrane proteins, which are expressed in chemosensory neurons that detect environmental stimuli. Here we identify homologues of GRs (Gustatory receptor-like (Grl) genes) in genomes across Protostomia, Deuterostomia and non-Bilateria. Surprisingly, two Grls in the cnidarian Nematostella vectensis, NvecGrl1 and NvecGrl2, are expressed early in development, in the blastula and gastrula, but not at later stages when a putative chemosensory organ forms. NvecGrl1 transcripts are detected around the arboral pole, considered the equivalent to the head-forming region of Bilateria. Morpholino-mediated knockdown of NvecGrl1 causes developmental patterning defects of this region, leading to animals lacking the apical sensory organ. A deuterostome Grl from the sea urchin Strongylocentrotus purpuratus displays similar patterns of developmental expression. These results reveal an early evolutionary origin of the insect chemosensory receptor family, and raise the possibility that their ancestral role was in embryonic development. 2015-02-18 /pmc/articles/PMC4374167/ /pubmed/25692633 http://dx.doi.org/10.1038/ncomms7243 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Saina, Michael Busengdal, Henriette Sinigaglia, Chiara Petrone, Libero Oliveri, Paola Rentzsch, Fabian Benton, Richard A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
title | A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
title_full | A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
title_fullStr | A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
title_full_unstemmed | A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
title_short | A cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
title_sort | cnidarian homologue of an insect gustatory receptor functions in developmental body patterning |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374167/ https://www.ncbi.nlm.nih.gov/pubmed/25692633 http://dx.doi.org/10.1038/ncomms7243 |
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