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Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender

PURPOSE: The aim of the study was to evaluate salivary flow rate, pH, buffering capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries, age and gender. MATERIALS AND METHODS: The study population consisted of 120 healthy children aged 7–15 years that was...

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Autores principales: Pandey, Pallavi, Reddy, N. Venugopal, Rao, V. Arun Prasad, Saxena, Aditya, Chaudhary, C. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374323/
https://www.ncbi.nlm.nih.gov/pubmed/25821379
http://dx.doi.org/10.4103/0976-237X.152943
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author Pandey, Pallavi
Reddy, N. Venugopal
Rao, V. Arun Prasad
Saxena, Aditya
Chaudhary, C. P.
author_facet Pandey, Pallavi
Reddy, N. Venugopal
Rao, V. Arun Prasad
Saxena, Aditya
Chaudhary, C. P.
author_sort Pandey, Pallavi
collection PubMed
description PURPOSE: The aim of the study was to evaluate salivary flow rate, pH, buffering capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries, age and gender. MATERIALS AND METHODS: The study population consisted of 120 healthy children aged 7–15 years that was further divided into two groups: 7–10 years and 11–15 years. In this 60 children with DMFS/dfs = 0 and 60 children with DMFS/dfs ≥5 were included. The subjects were divided into two groups; Group A: Children with DMFS/dfs = 0 (caries-free) Group B: Children with DMFS/dfs ≥5 (caries active). Unstimulated saliva samples were collected from all groups. Flow rates were determined, and samples analyzed for pH, buffer capacity, calcium, total protein and total antioxidant status. Salivary antioxidant activity is measured with spectrophotometer by an adaptation of 2,2’-azino-di-(3-ethylbenzthiazoline-6-sulphonate) assays. RESULTS: The mean difference of the two groups; caries-free and caries active were proved to be statistically significant (P < 0.05) for salivary calcium, total protein and total antioxidant level for both the sexes in the age group 7–10 years and for the age 11–15 years the mean difference of the two groups were proved to be statistically significant (P < 0.05) for salivary calcium level for both the sexes. Salivary total protein and total antioxidant level were proved to be statistically significant for male children only. CONCLUSIONS: In general, total protein and total antioxidants in saliva were increased with caries activity. Calcium content of saliva was found to be more in caries-free group and increased with age.
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spelling pubmed-43743232015-03-27 Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender Pandey, Pallavi Reddy, N. Venugopal Rao, V. Arun Prasad Saxena, Aditya Chaudhary, C. P. Contemp Clin Dent Original Article PURPOSE: The aim of the study was to evaluate salivary flow rate, pH, buffering capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries, age and gender. MATERIALS AND METHODS: The study population consisted of 120 healthy children aged 7–15 years that was further divided into two groups: 7–10 years and 11–15 years. In this 60 children with DMFS/dfs = 0 and 60 children with DMFS/dfs ≥5 were included. The subjects were divided into two groups; Group A: Children with DMFS/dfs = 0 (caries-free) Group B: Children with DMFS/dfs ≥5 (caries active). Unstimulated saliva samples were collected from all groups. Flow rates were determined, and samples analyzed for pH, buffer capacity, calcium, total protein and total antioxidant status. Salivary antioxidant activity is measured with spectrophotometer by an adaptation of 2,2’-azino-di-(3-ethylbenzthiazoline-6-sulphonate) assays. RESULTS: The mean difference of the two groups; caries-free and caries active were proved to be statistically significant (P < 0.05) for salivary calcium, total protein and total antioxidant level for both the sexes in the age group 7–10 years and for the age 11–15 years the mean difference of the two groups were proved to be statistically significant (P < 0.05) for salivary calcium level for both the sexes. Salivary total protein and total antioxidant level were proved to be statistically significant for male children only. CONCLUSIONS: In general, total protein and total antioxidants in saliva were increased with caries activity. Calcium content of saliva was found to be more in caries-free group and increased with age. Medknow Publications & Media Pvt Ltd 2015-03 /pmc/articles/PMC4374323/ /pubmed/25821379 http://dx.doi.org/10.4103/0976-237X.152943 Text en Copyright: © Contemporary Clinical Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pandey, Pallavi
Reddy, N. Venugopal
Rao, V. Arun Prasad
Saxena, Aditya
Chaudhary, C. P.
Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
title Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
title_full Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
title_fullStr Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
title_full_unstemmed Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
title_short Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
title_sort estimation of salivary flow rate, ph, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374323/
https://www.ncbi.nlm.nih.gov/pubmed/25821379
http://dx.doi.org/10.4103/0976-237X.152943
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