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An inducible recA expression Bacillus subtilis genome vector for stable manipulation of large DNA fragments

BACKGROUND: The Bacillus subtilis genome (BGM) vector is a novel cloning system based on the natural competence that enables B. subtilis to import extracellular DNA fragments into the cell and incorporate the recombinogenic DNA into the genome vector by homologous recombination. The BGM vector syste...

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Detalles Bibliográficos
Autores principales: Ogawa, Takafumi, Iwata, Tetsuo, Kaneko, Shinya, Itaya, Mitsuhiro, Hirota, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374399/
https://www.ncbi.nlm.nih.gov/pubmed/25879542
http://dx.doi.org/10.1186/s12864-015-1425-4