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An inducible recA expression Bacillus subtilis genome vector for stable manipulation of large DNA fragments
BACKGROUND: The Bacillus subtilis genome (BGM) vector is a novel cloning system based on the natural competence that enables B. subtilis to import extracellular DNA fragments into the cell and incorporate the recombinogenic DNA into the genome vector by homologous recombination. The BGM vector syste...
Autores principales: | Ogawa, Takafumi, Iwata, Tetsuo, Kaneko, Shinya, Itaya, Mitsuhiro, Hirota, Junji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374399/ https://www.ncbi.nlm.nih.gov/pubmed/25879542 http://dx.doi.org/10.1186/s12864-015-1425-4 |
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