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Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model

The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120±5.0 g) were anest...

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Autores principales: Manrique, Wilson Gómez, da Silva Claudiano, Gustavo, de Castro, Marcello Pardi, Petrillo, Thalita Regina, Figueiredo, Mayra Araguaia Pereira, de Andrade Belo, Marco Antonio, Berdeal, María Isabel Quiroga, de Moraes, Julieta Engracia Rodini, de Moraes, Flávio Ruas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374665/
https://www.ncbi.nlm.nih.gov/pubmed/25811875
http://dx.doi.org/10.1371/journal.pone.0121625
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author Manrique, Wilson Gómez
da Silva Claudiano, Gustavo
de Castro, Marcello Pardi
Petrillo, Thalita Regina
Figueiredo, Mayra Araguaia Pereira
de Andrade Belo, Marco Antonio
Berdeal, María Isabel Quiroga
de Moraes, Julieta Engracia Rodini
de Moraes, Flávio Ruas
author_facet Manrique, Wilson Gómez
da Silva Claudiano, Gustavo
de Castro, Marcello Pardi
Petrillo, Thalita Regina
Figueiredo, Mayra Araguaia Pereira
de Andrade Belo, Marco Antonio
Berdeal, María Isabel Quiroga
de Moraes, Julieta Engracia Rodini
de Moraes, Flávio Ruas
author_sort Manrique, Wilson Gómez
collection PubMed
description The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120±5.0 g) were anesthetized and 45 inoculated with 20 μL (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3(rd), 7(th), 14(th), 21(st) and 33(rd) days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14(th) to the 33(rd) day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14(th) DPI, and the stronger immunostaining in the 33(rd) day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14(th) day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33(rd) DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction.
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spelling pubmed-43746652015-04-04 Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model Manrique, Wilson Gómez da Silva Claudiano, Gustavo de Castro, Marcello Pardi Petrillo, Thalita Regina Figueiredo, Mayra Araguaia Pereira de Andrade Belo, Marco Antonio Berdeal, María Isabel Quiroga de Moraes, Julieta Engracia Rodini de Moraes, Flávio Ruas PLoS One Research Article The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120±5.0 g) were anesthetized and 45 inoculated with 20 μL (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3(rd), 7(th), 14(th), 21(st) and 33(rd) days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14(th) to the 33(rd) day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14(th) DPI, and the stronger immunostaining in the 33(rd) day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14(th) day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33(rd) DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction. Public Library of Science 2015-03-26 /pmc/articles/PMC4374665/ /pubmed/25811875 http://dx.doi.org/10.1371/journal.pone.0121625 Text en © 2015 Manrique et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Manrique, Wilson Gómez
da Silva Claudiano, Gustavo
de Castro, Marcello Pardi
Petrillo, Thalita Regina
Figueiredo, Mayra Araguaia Pereira
de Andrade Belo, Marco Antonio
Berdeal, María Isabel Quiroga
de Moraes, Julieta Engracia Rodini
de Moraes, Flávio Ruas
Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
title Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
title_full Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
title_fullStr Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
title_full_unstemmed Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
title_short Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
title_sort expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374665/
https://www.ncbi.nlm.nih.gov/pubmed/25811875
http://dx.doi.org/10.1371/journal.pone.0121625
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