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Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model
The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120±5.0 g) were anest...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374665/ https://www.ncbi.nlm.nih.gov/pubmed/25811875 http://dx.doi.org/10.1371/journal.pone.0121625 |
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author | Manrique, Wilson Gómez da Silva Claudiano, Gustavo de Castro, Marcello Pardi Petrillo, Thalita Regina Figueiredo, Mayra Araguaia Pereira de Andrade Belo, Marco Antonio Berdeal, María Isabel Quiroga de Moraes, Julieta Engracia Rodini de Moraes, Flávio Ruas |
author_facet | Manrique, Wilson Gómez da Silva Claudiano, Gustavo de Castro, Marcello Pardi Petrillo, Thalita Regina Figueiredo, Mayra Araguaia Pereira de Andrade Belo, Marco Antonio Berdeal, María Isabel Quiroga de Moraes, Julieta Engracia Rodini de Moraes, Flávio Ruas |
author_sort | Manrique, Wilson Gómez |
collection | PubMed |
description | The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120±5.0 g) were anesthetized and 45 inoculated with 20 μL (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3(rd), 7(th), 14(th), 21(st) and 33(rd) days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14(th) to the 33(rd) day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14(th) DPI, and the stronger immunostaining in the 33(rd) day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14(th) day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33(rd) DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction. |
format | Online Article Text |
id | pubmed-4374665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43746652015-04-04 Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model Manrique, Wilson Gómez da Silva Claudiano, Gustavo de Castro, Marcello Pardi Petrillo, Thalita Regina Figueiredo, Mayra Araguaia Pereira de Andrade Belo, Marco Antonio Berdeal, María Isabel Quiroga de Moraes, Julieta Engracia Rodini de Moraes, Flávio Ruas PLoS One Research Article The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120±5.0 g) were anesthetized and 45 inoculated with 20 μL (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3(rd), 7(th), 14(th), 21(st) and 33(rd) days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14(th) to the 33(rd) day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14(th) DPI, and the stronger immunostaining in the 33(rd) day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14(th) day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33(rd) DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction. Public Library of Science 2015-03-26 /pmc/articles/PMC4374665/ /pubmed/25811875 http://dx.doi.org/10.1371/journal.pone.0121625 Text en © 2015 Manrique et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Manrique, Wilson Gómez da Silva Claudiano, Gustavo de Castro, Marcello Pardi Petrillo, Thalita Regina Figueiredo, Mayra Araguaia Pereira de Andrade Belo, Marco Antonio Berdeal, María Isabel Quiroga de Moraes, Julieta Engracia Rodini de Moraes, Flávio Ruas Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model |
title | Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model |
title_full | Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model |
title_fullStr | Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model |
title_full_unstemmed | Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model |
title_short | Expression of Cellular Components in Granulomatous Inflammatory Response in Piaractus mesopotamicus Model |
title_sort | expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374665/ https://www.ncbi.nlm.nih.gov/pubmed/25811875 http://dx.doi.org/10.1371/journal.pone.0121625 |
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