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A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms
Bovine mastitis is a major threat to animal health and the dairy industry. Staphylococcus aureus is a contagious pathogen that is usually associated with persistent intramammary infections, and biofilm formation is a relevant aspect of the outcome of these infections. Several biological activities h...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374669/ https://www.ncbi.nlm.nih.gov/pubmed/25811661 http://dx.doi.org/10.1371/journal.pone.0120514 |
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author | Klein, Raphael Contelli Fabres-Klein, Mary Hellen de Oliveira, Leandro Licursi Feio, Renato Neves Malouin, François Ribon, Andréa de Oliveira Barros |
author_facet | Klein, Raphael Contelli Fabres-Klein, Mary Hellen de Oliveira, Leandro Licursi Feio, Renato Neves Malouin, François Ribon, Andréa de Oliveira Barros |
author_sort | Klein, Raphael Contelli |
collection | PubMed |
description | Bovine mastitis is a major threat to animal health and the dairy industry. Staphylococcus aureus is a contagious pathogen that is usually associated with persistent intramammary infections, and biofilm formation is a relevant aspect of the outcome of these infections. Several biological activities have been described for snake venoms, which led us to screen secretions of Bothrops jararacussu for antibiofilm activity against S. aureus NRS155. Crude venom was fractionated by size-exclusion chromatography, and the fractions were tested against S. aureus. Biofilm growth, but not bacterial growth, was affected by several fractions. Two fractions (15 and 16) showed the best activities and were also assayed against S. epidermidis NRS101. Fraction 15 was identified by TripleTOF mass spectrometry as a galactose-binding C-type lectin with a molecular weight of 15 kDa. The lectin was purified from the crude venom by D-galactose affinity chromatography, and only one peak was observed. This pure lectin was able to inhibit 75% and 80% of S. aureus and S. epidermidis biofilms, respectively, without affecting bacterial cell viability. The lectin also exhibited a dose-dependent inhibitory effect on both bacterial biofilms. The antibiofilm activity was confirmed using scanning electron microscopy. A pre-formed S. epidermidis biofilm was significantly disrupted by the C-type lectin in a time-dependent manner. Additionally, the lectin demonstrated the ability to inhibit biofilm formation by several mastitis pathogens, including different field strains of S. aureus, S. hyicus, S. chromogenes, Streptococcus agalactiae, and Escherichia coli. These findings reveal a new activity for C-type lectins. Studies are underway to evaluate the biological activity of these lectins in a mouse mastitis model. |
format | Online Article Text |
id | pubmed-4374669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43746692015-04-04 A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms Klein, Raphael Contelli Fabres-Klein, Mary Hellen de Oliveira, Leandro Licursi Feio, Renato Neves Malouin, François Ribon, Andréa de Oliveira Barros PLoS One Research Article Bovine mastitis is a major threat to animal health and the dairy industry. Staphylococcus aureus is a contagious pathogen that is usually associated with persistent intramammary infections, and biofilm formation is a relevant aspect of the outcome of these infections. Several biological activities have been described for snake venoms, which led us to screen secretions of Bothrops jararacussu for antibiofilm activity against S. aureus NRS155. Crude venom was fractionated by size-exclusion chromatography, and the fractions were tested against S. aureus. Biofilm growth, but not bacterial growth, was affected by several fractions. Two fractions (15 and 16) showed the best activities and were also assayed against S. epidermidis NRS101. Fraction 15 was identified by TripleTOF mass spectrometry as a galactose-binding C-type lectin with a molecular weight of 15 kDa. The lectin was purified from the crude venom by D-galactose affinity chromatography, and only one peak was observed. This pure lectin was able to inhibit 75% and 80% of S. aureus and S. epidermidis biofilms, respectively, without affecting bacterial cell viability. The lectin also exhibited a dose-dependent inhibitory effect on both bacterial biofilms. The antibiofilm activity was confirmed using scanning electron microscopy. A pre-formed S. epidermidis biofilm was significantly disrupted by the C-type lectin in a time-dependent manner. Additionally, the lectin demonstrated the ability to inhibit biofilm formation by several mastitis pathogens, including different field strains of S. aureus, S. hyicus, S. chromogenes, Streptococcus agalactiae, and Escherichia coli. These findings reveal a new activity for C-type lectins. Studies are underway to evaluate the biological activity of these lectins in a mouse mastitis model. Public Library of Science 2015-03-26 /pmc/articles/PMC4374669/ /pubmed/25811661 http://dx.doi.org/10.1371/journal.pone.0120514 Text en © 2015 Klein et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Klein, Raphael Contelli Fabres-Klein, Mary Hellen de Oliveira, Leandro Licursi Feio, Renato Neves Malouin, François Ribon, Andréa de Oliveira Barros A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms |
title | A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms |
title_full | A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms |
title_fullStr | A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms |
title_full_unstemmed | A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms |
title_short | A C-Type Lectin from Bothrops jararacussu Venom Disrupts Staphylococcal Biofilms |
title_sort | c-type lectin from bothrops jararacussu venom disrupts staphylococcal biofilms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374669/ https://www.ncbi.nlm.nih.gov/pubmed/25811661 http://dx.doi.org/10.1371/journal.pone.0120514 |
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