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Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

Open cell-free translation systems based on Escherichia coli cell lysates have successfully been used to produce antibodies and antibody fragments. In this study, we demonstrate the cell-free expression of functional single-chain antibody variable fragments (scFvs) in a eukaryotic and endotoxin-free...

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Detalles Bibliográficos
Autores principales: Stech, Marlitt, Hust, Michael, Schulze, Corina, Dübel, Stefan, Kubick, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374706/
https://www.ncbi.nlm.nih.gov/pubmed/25821419
http://dx.doi.org/10.1002/elsc.201400036