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Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments
Open cell-free translation systems based on Escherichia coli cell lysates have successfully been used to produce antibodies and antibody fragments. In this study, we demonstrate the cell-free expression of functional single-chain antibody variable fragments (scFvs) in a eukaryotic and endotoxin-free...
Autores principales: | Stech, Marlitt, Hust, Michael, Schulze, Corina, Dübel, Stefan, Kubick, Stefan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374706/ https://www.ncbi.nlm.nih.gov/pubmed/25821419 http://dx.doi.org/10.1002/elsc.201400036 |
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