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A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.

Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previousl...

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Autores principales: Cellier, Gilles, Moreau, Aurélie, Chabirand, Aude, Hostachy, Bruno, Ailloud, Florent, Prior, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374791/
https://www.ncbi.nlm.nih.gov/pubmed/25811378
http://dx.doi.org/10.1371/journal.pone.0122182
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author Cellier, Gilles
Moreau, Aurélie
Chabirand, Aude
Hostachy, Bruno
Ailloud, Florent
Prior, Philippe
author_facet Cellier, Gilles
Moreau, Aurélie
Chabirand, Aude
Hostachy, Bruno
Ailloud, Florent
Prior, Philippe
author_sort Cellier, Gilles
collection PubMed
description Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko strains are paraphyletic and are distributed among seven related but distinct phylogenetic clusters (sequevars) that are potentially major threats to Musaceae, Solanaceae, and ornamental crops in many countries. Although clustered within the Moko IIB-4 sequevar, strains of the epidemiologically variant IIB-4NPB do not cause wilt on Cavendish or plantain bananas; instead, they establish a latent infection in the vascular tissues of plantains and demonstrate an expanded host range and high aggressiveness toward Solanaceae and Cucurbitaceae. Although most molecular diagnostic methods focus on strains that wilt Solanaceae (particularly potato), no relevant protocol has been described that universally detects strains of the Musaceae-infecting Rs phylotype II. Thus, a duplex PCR assay targeting Moko and IIB-4NPB variant strains was developed, and its performance was assessed using an extensive collection of 111 strains representing the known diversity of Rs Moko-related strains and IIB-4NPB variant strains along with certain related strains and families. The proposed diagnostic protocol demonstrated both high accuracy (inclusivity and exclusivity) and high repeatability, detected targets on either pure culture or spiked plant extracts. Although they did not belong to the Moko clusters described at the time of the study, recently discovered banana-infecting strains from Brazil were also detected. According to our comprehensive evaluation, this duplex PCR assay appears suitable for both research and diagnostic laboratories and provides reliable detection of phylotype II Rs strains that infect Musaceae.
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spelling pubmed-43747912015-04-04 A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp. Cellier, Gilles Moreau, Aurélie Chabirand, Aude Hostachy, Bruno Ailloud, Florent Prior, Philippe PLoS One Research Article Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko strains are paraphyletic and are distributed among seven related but distinct phylogenetic clusters (sequevars) that are potentially major threats to Musaceae, Solanaceae, and ornamental crops in many countries. Although clustered within the Moko IIB-4 sequevar, strains of the epidemiologically variant IIB-4NPB do not cause wilt on Cavendish or plantain bananas; instead, they establish a latent infection in the vascular tissues of plantains and demonstrate an expanded host range and high aggressiveness toward Solanaceae and Cucurbitaceae. Although most molecular diagnostic methods focus on strains that wilt Solanaceae (particularly potato), no relevant protocol has been described that universally detects strains of the Musaceae-infecting Rs phylotype II. Thus, a duplex PCR assay targeting Moko and IIB-4NPB variant strains was developed, and its performance was assessed using an extensive collection of 111 strains representing the known diversity of Rs Moko-related strains and IIB-4NPB variant strains along with certain related strains and families. The proposed diagnostic protocol demonstrated both high accuracy (inclusivity and exclusivity) and high repeatability, detected targets on either pure culture or spiked plant extracts. Although they did not belong to the Moko clusters described at the time of the study, recently discovered banana-infecting strains from Brazil were also detected. According to our comprehensive evaluation, this duplex PCR assay appears suitable for both research and diagnostic laboratories and provides reliable detection of phylotype II Rs strains that infect Musaceae. Public Library of Science 2015-03-26 /pmc/articles/PMC4374791/ /pubmed/25811378 http://dx.doi.org/10.1371/journal.pone.0122182 Text en © 2015 Cellier et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cellier, Gilles
Moreau, Aurélie
Chabirand, Aude
Hostachy, Bruno
Ailloud, Florent
Prior, Philippe
A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
title A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
title_full A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
title_fullStr A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
title_full_unstemmed A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
title_short A Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
title_sort duplex pcr assay for the detection of ralstonia solanacearum phylotype ii strains in musa spp.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374791/
https://www.ncbi.nlm.nih.gov/pubmed/25811378
http://dx.doi.org/10.1371/journal.pone.0122182
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