Cargando…

Effect of Phosphorylation on EGFR Dimer Stability Probed by Single-Molecule Dynamics and FRET/FLIM

Deregulation of epidermal growth factor receptor (EGFR) signaling has been correlated with the development of a variety of human carcinomas. EGF-induced receptor dimerization and consequent trans- auto-phosphorylation are among the earliest events in signal transduction. Binding of EGF is thought to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Coban, Oana, Zanetti-Dominguez, Laura C., Matthews, Daniel R., Rolfe, Daniel J., Weitsman, Gregory, Barber, Paul R., Barbeau, Jody, Devauges, Viviane, Kampmeier, Florian, Winn, Martyn, Vojnovic, Borivoj, Parker, Peter J., Lidke, Keith A., Lidke, Diane S., Ameer-Beg, Simon M., Martin-Fernandez, Marisa L., Ng, Tony
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Biophysical Society 2015
Materias:	Cell Biophysics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375452/ https://www.ncbi.nlm.nih.gov/pubmed/25762314 http://dx.doi.org/10.1016/j.bpj.2015.01.005

Ejemplares similares

Adaptive optics for a time-resolved Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) in vivo
por: Coelho, Simao, et al.
Publicado: (2020)

Steady-State Acceptor Fluorescence Anisotropy Imaging under Evanescent Excitation for Visualisation of FRET at the Plasma Membrane
por: Devauges, Viviane, et al.
Publicado: (2014)

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
por: Waterhouse, Benjamin R., et al.
Publicado: (2011)

HER2-HER3 Heterodimer Quantification by FRET-FLIM and Patient Subclass Analysis of the COIN Colorectal Trial
por: Barber, Paul R, et al.
Publicado: (2019)

HER2-HER3 dimer quantification by FLIM-FRET predicts breast cancer metastatic relapse independently of HER2 IHC status
por: Weitsman, Gregory, et al.
Publicado: (2016)

Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM
por: Levitt, James A., et al.
Publicado: (2020)

Robust Bayesian Fluorescence Lifetime Estimation, Decay Model Selection and Instrument Response Determination for Low-Intensity FLIM Imaging
por: Rowley, Mark I., et al.
Publicado: (2016)

Correction: Robust Bayesian Fluorescence Lifetime Estimation, Decay Model Selection and Instrument Response Determination for Low-Intensity FLIM Imaging
por: Rowley, Mark I., et al.
Publicado: (2016)

Practical and reliable FRET/FLIM pair of fluorescent proteins
por: Shcherbo, Dmitry, et al.
Publicado: (2009)

A Bayesian method for single molecule, fluorescence burst analysis
por: Barber, P. R., et al.
Publicado: (2010)

FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis
por: Kim, Jiho, et al.
Publicado: (2017)

Integrating single-molecule FRET and biomolecular simulations to study diverse interactions between nucleic acids and proteins
por: Sanders, Joshua C., et al.
Publicado: (2021)

Dynamic FRET-FLIM based screening of signal transduction pathways
por: Harkes, Rolf, et al.
Publicado: (2021)

A Multi-Functional Imaging Approach to High-Content Protein Interaction Screening
por: Matthews, Daniel R., et al.
Publicado: (2012)

Quantitative analysis of chromatin compaction in living cells using FLIM–FRET
por: Llères, David, et al.
Publicado: (2009)

An Improved Ras Sensor for Highly Sensitive and Quantitative FRET-FLIM Imaging
por: Oliveira, Ana F., et al.
Publicado: (2013)

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
por: Ishikawa-Ankerhold, Hellen C., et al.
Publicado: (2012)

Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer
por: Liu, Wenjie, et al.
Publicado: (2019)

The in vivo mechanics of the magnetotactic backbone as revealed by correlative FLIM-FRET and STED microscopy
por: Günther, Erika, et al.
Publicado: (2019)

Using FLIM-FRET to Measure Conformational Changes of Transglutaminase Type 2 in Live Cells
por: Caron, Nicholas S., et al.
Publicado: (2012)

Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging
por: Omer, Travis, et al.
Publicado: (2015)

Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM
por: Chennell, George, et al.
Publicado: (2016)

ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement
por: Murakoshi, Hideji, et al.
Publicado: (2017)

Multi-target immunofluorescence by separation of antibody cross-labelling via spectral-FLIM-FRET
por: Rohilla, Sumeet, et al.
Publicado: (2020)

A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth
por: Chen, WeiYue, et al.
Publicado: (2015)

Single Cell FRET Analysis for the Identification of Optimal FRET-Pairs in Bacillus subtilis Using a Prototype MEM-FLIM System
por: Detert Oude Weme, Ruud G. J., et al.
Publicado: (2015)

Allosteric changes in HDM2 by the ATM phosphomimetic S395D mutation: implications on HDM2 function
por: Uhrik, Lukas, et al.
Publicado: (2019)

Molecular determinants of ER–Golgi contacts identified through a new FRET–FLIM system
por: Venditti, Rossella, et al.
Publicado: (2019)

Quantitative FRET-FLIM-BlaM to Assess the Extent of HIV-1 Fusion in Live Cells
por: Carlon-Andres, Irene, et al.
Publicado: (2020)

Cryptic in vitro ubiquitin ligase activity of HDMX towards p53 is probably regulated by an induced fit mechanism
por: Calderon-González, Karla Gisel, et al.
Publicado: (2022)

DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning
por: Thomsen, Johannes, et al.
Publicado: (2020)

FLIM-FRET Imaging of Caspase-3 Activity in Live Cells Using Pair of Red Fluorescent Proteins
por: Savitsky, Alexander P., et al.
Publicado: (2012)

Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles
por: Coucke, Quinten, et al.
Publicado: (2023)

FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ**
por: Kumar, Sunil, et al.
Publicado: (2011)

Multisite EGFR phosphorylation is regulated by adaptor protein abundances and dimer lifetimes
por: Salazar-Cavazos, Emanuel, et al.
Publicado: (2020)

FLIM FRET Visualization of Cdc42 Activation by Netrin-1 in Embryonic Spinal Commissural Neuron Growth Cones
por: Rappaz, Benjamin, et al.
Publicado: (2016)

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response
por: Lou, Jieqiong, et al.
Publicado: (2019)

Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
por: Aktalay, Ayse, et al.
Publicado: (2022)

Quantitative cell biology: uniting disciplines to understand the cell
por: Lidke, Diane S.
Publicado: (2017)

Sequential Superresolution Imaging of Multiple Targets Using a Single Fluorophore
por: Valley, Christopher C., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_oij7tsodonb0mb94n5n5cec00n