A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()

We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within...

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Detalles Bibliográficos
Autores principales: Bell, Laurence, Seshia, Ashwin, Lando, David, Laue, Ernest, Palayret, Matthieu, Lee, Steven F., Klenerman, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Sequoia 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375559/
https://www.ncbi.nlm.nih.gov/pubmed/25844024
http://dx.doi.org/10.1016/j.snb.2013.10.002
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author Bell, Laurence
Seshia, Ashwin
Lando, David
Laue, Ernest
Palayret, Matthieu
Lee, Steven F.
Klenerman, David
author_facet Bell, Laurence
Seshia, Ashwin
Lando, David
Laue, Ernest
Palayret, Matthieu
Lee, Steven F.
Klenerman, David
author_sort Bell, Laurence
collection PubMed
description We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
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spelling pubmed-43755592015-04-01 A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging() Bell, Laurence Seshia, Ashwin Lando, David Laue, Ernest Palayret, Matthieu Lee, Steven F. Klenerman, David Sens Actuators B Chem Article We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging. Elsevier Sequoia 2014-03-01 /pmc/articles/PMC4375559/ /pubmed/25844024 http://dx.doi.org/10.1016/j.snb.2013.10.002 Text en © 2013 The Authors https://creativecommons.org/licenses/by/3.0/This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Bell, Laurence
Seshia, Ashwin
Lando, David
Laue, Ernest
Palayret, Matthieu
Lee, Steven F.
Klenerman, David
A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
title A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
title_full A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
title_fullStr A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
title_full_unstemmed A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
title_short A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
title_sort microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375559/
https://www.ncbi.nlm.nih.gov/pubmed/25844024
http://dx.doi.org/10.1016/j.snb.2013.10.002
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