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Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer
BACKGROUND: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-D...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375847/ https://www.ncbi.nlm.nih.gov/pubmed/25885025 http://dx.doi.org/10.1186/s12864-015-1363-1 |
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author | Zhang, Yi Tong Zhang, Yu Liang Chen, Si Xue Yin, Guo Hua Yang, Ze Zhong Lee, Samantha Liu, Chun Guang Zhao, Dan Dan Ma, Yu Kun Song, Fu Qiang Bennett, Joan W Yang, Feng Shan |
author_facet | Zhang, Yi Tong Zhang, Yu Liang Chen, Si Xue Yin, Guo Hua Yang, Ze Zhong Lee, Samantha Liu, Chun Guang Zhao, Dan Dan Ma, Yu Kun Song, Fu Qiang Bennett, Joan W Yang, Feng Shan |
author_sort | Zhang, Yi Tong |
collection | PubMed |
description | BACKGROUND: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-D gel, we identified 62 MeJA-responsive proteins and measured protein expression level changes. RESULTS: Among these 62 proteins, 43 proteins levels were increased while 11 proteins were decreased. We also found eight proteins uniquely expressed in response to MeJA treatment. Data are available via ProteomeXchange with identifier PXD001793. The proteins identified in this study have important biological functions including photosynthesis and energy related proteins (38.4%), protein folding, degradation and regulated proteins (15.0%), stress and defense regulated proteins (11.7%), and redox-responsive proteins (8.3%). The expression levels of four important genes were determined by qRT-PCR analysis. The expression levels of these proteins did not correlate well with their translation levels. To test the defense functions of the differentially expressed proteins, expression vectors of four protein coding genes were constructed to express in-fusion proteins in E. coli. The expressed proteins were used to feed Ostrinia furnacalis, the Asian corn borer (ACB). Our results demonstrated that the recombinant proteins of pathogenesis-related protein 1 (PR1) and thioredoxin M-type, chloroplastic precursor (TRXM) showed the significant inhibition on the development of larvae and pupae. CONCLUSIONS: We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1363-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4375847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43758472015-03-28 Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer Zhang, Yi Tong Zhang, Yu Liang Chen, Si Xue Yin, Guo Hua Yang, Ze Zhong Lee, Samantha Liu, Chun Guang Zhao, Dan Dan Ma, Yu Kun Song, Fu Qiang Bennett, Joan W Yang, Feng Shan BMC Genomics Research Article BACKGROUND: Jasmonic acid (JA) and methyl jasmonate (MeJA) regulate plant development, resistance to stress, and insect attack by inducing specific gene expression. However, little is known about the mechanism of plant defense against herbivore attack at a protein level. Using a high-resolution 2-D gel, we identified 62 MeJA-responsive proteins and measured protein expression level changes. RESULTS: Among these 62 proteins, 43 proteins levels were increased while 11 proteins were decreased. We also found eight proteins uniquely expressed in response to MeJA treatment. Data are available via ProteomeXchange with identifier PXD001793. The proteins identified in this study have important biological functions including photosynthesis and energy related proteins (38.4%), protein folding, degradation and regulated proteins (15.0%), stress and defense regulated proteins (11.7%), and redox-responsive proteins (8.3%). The expression levels of four important genes were determined by qRT-PCR analysis. The expression levels of these proteins did not correlate well with their translation levels. To test the defense functions of the differentially expressed proteins, expression vectors of four protein coding genes were constructed to express in-fusion proteins in E. coli. The expressed proteins were used to feed Ostrinia furnacalis, the Asian corn borer (ACB). Our results demonstrated that the recombinant proteins of pathogenesis-related protein 1 (PR1) and thioredoxin M-type, chloroplastic precursor (TRXM) showed the significant inhibition on the development of larvae and pupae. CONCLUSIONS: We found MeJA could not only induce plant defense mechanisms to insects, it also enhanced toxic protein production that potentially can be used for bio-control of ACB. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1363-1) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-21 /pmc/articles/PMC4375847/ /pubmed/25885025 http://dx.doi.org/10.1186/s12864-015-1363-1 Text en © Zhang et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zhang, Yi Tong Zhang, Yu Liang Chen, Si Xue Yin, Guo Hua Yang, Ze Zhong Lee, Samantha Liu, Chun Guang Zhao, Dan Dan Ma, Yu Kun Song, Fu Qiang Bennett, Joan W Yang, Feng Shan Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer |
title | Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer |
title_full | Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer |
title_fullStr | Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer |
title_full_unstemmed | Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer |
title_short | Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer |
title_sort | proteomics of methyl jasmonate induced defense response in maize leaves against asian corn borer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375847/ https://www.ncbi.nlm.nih.gov/pubmed/25885025 http://dx.doi.org/10.1186/s12864-015-1363-1 |
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