Structural Phenotyping of Stem Cell-Derived Cardiomyocytes

Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs int...

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Detalles Bibliográficos
Autores principales: Pasqualini, Francesco Silvio, Sheehy, Sean Paul, Agarwal, Ashutosh, Aratyn-Schaus, Yvonne, Parker, Kevin Kit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375945/
https://www.ncbi.nlm.nih.gov/pubmed/25733020
http://dx.doi.org/10.1016/j.stemcr.2015.01.020
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author Pasqualini, Francesco Silvio
Sheehy, Sean Paul
Agarwal, Ashutosh
Aratyn-Schaus, Yvonne
Parker, Kevin Kit
author_facet Pasqualini, Francesco Silvio
Sheehy, Sean Paul
Agarwal, Ashutosh
Aratyn-Schaus, Yvonne
Parker, Kevin Kit
author_sort Pasqualini, Francesco Silvio
collection PubMed
description Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.
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spelling pubmed-43759452015-04-03 Structural Phenotyping of Stem Cell-Derived Cardiomyocytes Pasqualini, Francesco Silvio Sheehy, Sean Paul Agarwal, Ashutosh Aratyn-Schaus, Yvonne Parker, Kevin Kit Stem Cell Reports Report Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes. Elsevier 2015-02-26 /pmc/articles/PMC4375945/ /pubmed/25733020 http://dx.doi.org/10.1016/j.stemcr.2015.01.020 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Report
Pasqualini, Francesco Silvio
Sheehy, Sean Paul
Agarwal, Ashutosh
Aratyn-Schaus, Yvonne
Parker, Kevin Kit
Structural Phenotyping of Stem Cell-Derived Cardiomyocytes
title Structural Phenotyping of Stem Cell-Derived Cardiomyocytes
title_full Structural Phenotyping of Stem Cell-Derived Cardiomyocytes
title_fullStr Structural Phenotyping of Stem Cell-Derived Cardiomyocytes
title_full_unstemmed Structural Phenotyping of Stem Cell-Derived Cardiomyocytes
title_short Structural Phenotyping of Stem Cell-Derived Cardiomyocytes
title_sort structural phenotyping of stem cell-derived cardiomyocytes
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375945/
https://www.ncbi.nlm.nih.gov/pubmed/25733020
http://dx.doi.org/10.1016/j.stemcr.2015.01.020
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