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Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study
BACKGROUND: Cryptosporidium infection is a worldwide cause of diarrheal disease. To gain insight into the epidemiology of the infection in a certain geographic area, molecular methods are needed to determine the species/genotypes and subtypes. METHODOLOGY/PRINCIPAL FINDINGS: From 2004 to 2009, 161 c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376526/ https://www.ncbi.nlm.nih.gov/pubmed/25816024 http://dx.doi.org/10.1371/journal.pone.0121753 |
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author | Segura, Remedios Prim, Núria Montemayor, Michel Valls, María Eugenia Muñoz, Carme |
author_facet | Segura, Remedios Prim, Núria Montemayor, Michel Valls, María Eugenia Muñoz, Carme |
author_sort | Segura, Remedios |
collection | PubMed |
description | BACKGROUND: Cryptosporidium infection is a worldwide cause of diarrheal disease. To gain insight into the epidemiology of the infection in a certain geographic area, molecular methods are needed to determine the species/genotypes and subtypes. METHODOLOGY/PRINCIPAL FINDINGS: From 2004 to 2009, 161 cryptosporidiosis cases were detected in two hospitals in Barcelona. Diagnosis was performed by microscopic observation of oocysts in stool specimens following modified Ziehl-Neelsen staining. Most cases (82%) occurred in children. The number of cases increased in summer and autumn. Molecular characterization of Cryptosporidium was performed in 69 specimens, and C. hominis and C. parvum were identified in 88.4% and 10.1% of the cases, respectively. C. meleagridis was detected in one specimen. Subtyping based on the gp60 polymorphism showed six subtypes, four C. hominis and two C. parvum. Subtype IbA10G2 was the most prevalent subtype corresponding to 90% of all C. hominis isolates. This is the first report on the distribution of specific Cryptosporidium subtypes from humans in Spain. CONCLUSIONS/SIGNIFICANCE: In our geographic area, the anthroponotic behavior of C. hominis, the lower infective dose, and the higher virulence of certain subtypes may contribute to the high incidence of human cryptosporidiosis caused by the IbA10G2 subtype. Further studies should include populations with asymptomatic shedding of the parasite. |
format | Online Article Text |
id | pubmed-4376526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43765262015-04-04 Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study Segura, Remedios Prim, Núria Montemayor, Michel Valls, María Eugenia Muñoz, Carme PLoS One Research Article BACKGROUND: Cryptosporidium infection is a worldwide cause of diarrheal disease. To gain insight into the epidemiology of the infection in a certain geographic area, molecular methods are needed to determine the species/genotypes and subtypes. METHODOLOGY/PRINCIPAL FINDINGS: From 2004 to 2009, 161 cryptosporidiosis cases were detected in two hospitals in Barcelona. Diagnosis was performed by microscopic observation of oocysts in stool specimens following modified Ziehl-Neelsen staining. Most cases (82%) occurred in children. The number of cases increased in summer and autumn. Molecular characterization of Cryptosporidium was performed in 69 specimens, and C. hominis and C. parvum were identified in 88.4% and 10.1% of the cases, respectively. C. meleagridis was detected in one specimen. Subtyping based on the gp60 polymorphism showed six subtypes, four C. hominis and two C. parvum. Subtype IbA10G2 was the most prevalent subtype corresponding to 90% of all C. hominis isolates. This is the first report on the distribution of specific Cryptosporidium subtypes from humans in Spain. CONCLUSIONS/SIGNIFICANCE: In our geographic area, the anthroponotic behavior of C. hominis, the lower infective dose, and the higher virulence of certain subtypes may contribute to the high incidence of human cryptosporidiosis caused by the IbA10G2 subtype. Further studies should include populations with asymptomatic shedding of the parasite. Public Library of Science 2015-03-27 /pmc/articles/PMC4376526/ /pubmed/25816024 http://dx.doi.org/10.1371/journal.pone.0121753 Text en © 2015 Segura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Segura, Remedios Prim, Núria Montemayor, Michel Valls, María Eugenia Muñoz, Carme Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study |
title | Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study |
title_full | Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study |
title_fullStr | Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study |
title_full_unstemmed | Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study |
title_short | Predominant Virulent IbA10G2 Subtype of Cryptosporidium hominis in Human Isolates in Barcelona: A Five-Year Study |
title_sort | predominant virulent iba10g2 subtype of cryptosporidium hominis in human isolates in barcelona: a five-year study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376526/ https://www.ncbi.nlm.nih.gov/pubmed/25816024 http://dx.doi.org/10.1371/journal.pone.0121753 |
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