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A Comprehensive Alanine-Scanning Mutagenesis Study Reveals Roles for Salt Bridges in the Structure and Activity of Pseudomonas aeruginosa Elastase

The relationship between salt bridges and stability/enzymatic activity is unclear. We studied this relationship by systematic alanine-scanning mutation analysis using the typical M4 family metalloprotease Pseudomonas aeruginosa elastase (PAE, also known as pseudolysin) as a model. Structural analysi...

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Detalles Bibliográficos
Autores principales:	Bian, Fei, Yue, Shousong, Peng, Zhenying, Zhang, Xiaowei, Chen, Gao, Yu, Jinhui, Xuan, Ning, Bi, Yuping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376888/ https://www.ncbi.nlm.nih.gov/pubmed/25815820 http://dx.doi.org/10.1371/journal.pone.0121108

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