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Role of marsupial tammar wallaby milk in lung maturation of pouch young

BACKGROUND: Marsupials such as the tammar wallaby (M.Eugenii) have a short gestation (29.3 days) and at birth the altricial young resembles a fetus, and the major development occurs postnatally while the young remains in the mother’s pouch. The essential functional factors for the maturation of the...

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Autores principales: Modepalli, Vengamanaidu, Hinds, Lyn A, Sharp, Julie A, Lefevre, Christophe, Nicholas, Kevin R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377010/
https://www.ncbi.nlm.nih.gov/pubmed/25888082
http://dx.doi.org/10.1186/s12861-015-0063-z
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author Modepalli, Vengamanaidu
Hinds, Lyn A
Sharp, Julie A
Lefevre, Christophe
Nicholas, Kevin R
author_facet Modepalli, Vengamanaidu
Hinds, Lyn A
Sharp, Julie A
Lefevre, Christophe
Nicholas, Kevin R
author_sort Modepalli, Vengamanaidu
collection PubMed
description BACKGROUND: Marsupials such as the tammar wallaby (M.Eugenii) have a short gestation (29.3 days) and at birth the altricial young resembles a fetus, and the major development occurs postnatally while the young remains in the mother’s pouch. The essential functional factors for the maturation of the neonate are provided by the milk which changes in composition progressively throughout lactation (300 days). Morphologically the lungs of tammar pouch young are immature at birth and the majority of their development occurs during the first 100 days of lactation. RESULTS: In this study mouse embryonic lungs (E-12) were cultured in media with tammar skim milk collected at key time points of lactation to identify factors involved in regulating postnatal lung maturation. Remarkably the embryonic lungs showed increased branching morphogenesis and this effect was restricted to milk collected at specific time points between approximately day 40 to 100 lactation. Further analysis to assess lung development showed a significant increase in the expression of marker genes Sp-C, Sp-B, Wnt-7b, BMP4 and Id2 in lung cultures incubated with milk collected at day 60. Similarly, day 60 milk specifically stimulated proliferation and elongation of lung mesenchymal cells that invaded matrigel. In addition, this milk stimulated proliferation of lung epithelium cells on matrigel, and the cells formed 3-dimensional acini with an extended lumen. CONCLUSIONS: This study has clearly demonstrated that tammar wallaby milk collected at specific times in early lactation contains bioactives that may have a significant role in lung maturation of pouch young. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-015-0063-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-43770102015-03-29 Role of marsupial tammar wallaby milk in lung maturation of pouch young Modepalli, Vengamanaidu Hinds, Lyn A Sharp, Julie A Lefevre, Christophe Nicholas, Kevin R BMC Dev Biol Research Article BACKGROUND: Marsupials such as the tammar wallaby (M.Eugenii) have a short gestation (29.3 days) and at birth the altricial young resembles a fetus, and the major development occurs postnatally while the young remains in the mother’s pouch. The essential functional factors for the maturation of the neonate are provided by the milk which changes in composition progressively throughout lactation (300 days). Morphologically the lungs of tammar pouch young are immature at birth and the majority of their development occurs during the first 100 days of lactation. RESULTS: In this study mouse embryonic lungs (E-12) were cultured in media with tammar skim milk collected at key time points of lactation to identify factors involved in regulating postnatal lung maturation. Remarkably the embryonic lungs showed increased branching morphogenesis and this effect was restricted to milk collected at specific time points between approximately day 40 to 100 lactation. Further analysis to assess lung development showed a significant increase in the expression of marker genes Sp-C, Sp-B, Wnt-7b, BMP4 and Id2 in lung cultures incubated with milk collected at day 60. Similarly, day 60 milk specifically stimulated proliferation and elongation of lung mesenchymal cells that invaded matrigel. In addition, this milk stimulated proliferation of lung epithelium cells on matrigel, and the cells formed 3-dimensional acini with an extended lumen. CONCLUSIONS: This study has clearly demonstrated that tammar wallaby milk collected at specific times in early lactation contains bioactives that may have a significant role in lung maturation of pouch young. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12861-015-0063-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-21 /pmc/articles/PMC4377010/ /pubmed/25888082 http://dx.doi.org/10.1186/s12861-015-0063-z Text en © Modepalli et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Modepalli, Vengamanaidu
Hinds, Lyn A
Sharp, Julie A
Lefevre, Christophe
Nicholas, Kevin R
Role of marsupial tammar wallaby milk in lung maturation of pouch young
title Role of marsupial tammar wallaby milk in lung maturation of pouch young
title_full Role of marsupial tammar wallaby milk in lung maturation of pouch young
title_fullStr Role of marsupial tammar wallaby milk in lung maturation of pouch young
title_full_unstemmed Role of marsupial tammar wallaby milk in lung maturation of pouch young
title_short Role of marsupial tammar wallaby milk in lung maturation of pouch young
title_sort role of marsupial tammar wallaby milk in lung maturation of pouch young
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377010/
https://www.ncbi.nlm.nih.gov/pubmed/25888082
http://dx.doi.org/10.1186/s12861-015-0063-z
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