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Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter
BACKGROUND: Reteplase is a mutant version of t-PA (tissue plasminogen activator) with prolonged half-life. In the present study, E. coli Top 10 bacteria were utilized in the production of reteplase, which is the nonglycosylated active domain of t-PA. Reteplase gene was ligated into pBAD/gIII plasmid...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
DOCS
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377059/ https://www.ncbi.nlm.nih.gov/pubmed/25866712 |