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Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter

BACKGROUND: Reteplase is a mutant version of t-PA (tissue plasminogen activator) with prolonged half-life. In the present study, E. coli Top 10 bacteria were utilized in the production of reteplase, which is the nonglycosylated active domain of t-PA. Reteplase gene was ligated into pBAD/gIII plasmid...

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Detalles Bibliográficos
Autores principales: Shafiee, Fatemeh, Moazen, Fatemeh, Rabbani, Mahammad, Mir Mohammad Sadeghi, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: DOCS 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377059/
https://www.ncbi.nlm.nih.gov/pubmed/25866712

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