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Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR
BACKGROUND: Legionellaceae contains Legionella genus with over 52 species and 64 serogroups. It is one of the most important causes of respiratory disease in human. More than 30% of hospital-acquired pneumonia is caused by Legionella. Ventilator-associated pneumonia (VAP) is an infection acquired in...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377168/ https://www.ncbi.nlm.nih.gov/pubmed/25834717 http://dx.doi.org/10.5812/jjm.15094 |
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author | Divan Khosroshahi, Nader Naserpour Farivar, Taghi Johari, Pouran |
author_facet | Divan Khosroshahi, Nader Naserpour Farivar, Taghi Johari, Pouran |
author_sort | Divan Khosroshahi, Nader |
collection | PubMed |
description | BACKGROUND: Legionellaceae contains Legionella genus with over 52 species and 64 serogroups. It is one of the most important causes of respiratory disease in human. More than 30% of hospital-acquired pneumonia is caused by Legionella. Ventilator-associated pneumonia (VAP) is an infection acquired in hospital wards, particularly in intensive care unit (ICU). This disease approximately affects 9% to 20% of intubated patients. Mortality in these patients varies between 8% and 76%. Legionella is one of the important factors for infection in intubated patients. OBJECTIVES: The present study was aimed to investigate the use of molecular methods in diagnosis of infection caused by Legionella pneumophila. MATERIALS AND METHODS: In this study, 109 samples of lung secretions collected from intubated patients admitted to ICU wards of four university hospitals in a three-month period were examined. Cultivation and Real time Polymerase Chain Reaction (PCR) methods were used to assess L. pneumophila colonization in these samples. RESULTS: Eleven samples had positive results using real time PCR analysis of 16s rRNA gene fragments specific for L. pneumophila, but according to culture method on specific buffered charcoal-yeast extract medium (BCYE), no positive cases were detected. Of the total positive cases, six were males, one female and four infants. The seven adults aged 40-65 years. CONCLUSIONS: Using molecular methods in diagnosis of infection caused by L. pneumophila has a great value because of its high specificity and rapid diagnosis potency. |
format | Online Article Text |
id | pubmed-4377168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-43771682015-04-01 Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR Divan Khosroshahi, Nader Naserpour Farivar, Taghi Johari, Pouran Jundishapur J Microbiol Research Article BACKGROUND: Legionellaceae contains Legionella genus with over 52 species and 64 serogroups. It is one of the most important causes of respiratory disease in human. More than 30% of hospital-acquired pneumonia is caused by Legionella. Ventilator-associated pneumonia (VAP) is an infection acquired in hospital wards, particularly in intensive care unit (ICU). This disease approximately affects 9% to 20% of intubated patients. Mortality in these patients varies between 8% and 76%. Legionella is one of the important factors for infection in intubated patients. OBJECTIVES: The present study was aimed to investigate the use of molecular methods in diagnosis of infection caused by Legionella pneumophila. MATERIALS AND METHODS: In this study, 109 samples of lung secretions collected from intubated patients admitted to ICU wards of four university hospitals in a three-month period were examined. Cultivation and Real time Polymerase Chain Reaction (PCR) methods were used to assess L. pneumophila colonization in these samples. RESULTS: Eleven samples had positive results using real time PCR analysis of 16s rRNA gene fragments specific for L. pneumophila, but according to culture method on specific buffered charcoal-yeast extract medium (BCYE), no positive cases were detected. Of the total positive cases, six were males, one female and four infants. The seven adults aged 40-65 years. CONCLUSIONS: Using molecular methods in diagnosis of infection caused by L. pneumophila has a great value because of its high specificity and rapid diagnosis potency. Kowsar 2015-03-21 /pmc/articles/PMC4377168/ /pubmed/25834717 http://dx.doi.org/10.5812/jjm.15094 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Divan Khosroshahi, Nader Naserpour Farivar, Taghi Johari, Pouran Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR |
title | Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR |
title_full | Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR |
title_fullStr | Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR |
title_full_unstemmed | Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR |
title_short | Identification of Legionella Pneumophila in Intubated Patients With TaqMan Real Time PCR |
title_sort | identification of legionella pneumophila in intubated patients with taqman real time pcr |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377168/ https://www.ncbi.nlm.nih.gov/pubmed/25834717 http://dx.doi.org/10.5812/jjm.15094 |
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