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MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats

MicroRNAs (miRNAs) are a group of endogenous noncoding RNAs that play important roles in many biological processes. This study aimed to check if miRNAs were involved in the response to acupuncture in rats. Microarray analysis was performed to compare the miRNA expression profiles of medulla in spont...

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Autores principales: Wang, Jia-You, Li, Hui, Ma, Chun-Mei, Wang, Jia-Lu, Lai, Xin-Sheng, Zhou, Shu-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377461/
https://www.ncbi.nlm.nih.gov/pubmed/25861353
http://dx.doi.org/10.1155/2015/204367
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author Wang, Jia-You
Li, Hui
Ma, Chun-Mei
Wang, Jia-Lu
Lai, Xin-Sheng
Zhou, Shu-Feng
author_facet Wang, Jia-You
Li, Hui
Ma, Chun-Mei
Wang, Jia-Lu
Lai, Xin-Sheng
Zhou, Shu-Feng
author_sort Wang, Jia-You
collection PubMed
description MicroRNAs (miRNAs) are a group of endogenous noncoding RNAs that play important roles in many biological processes. This study aimed to check if miRNAs were involved in the response to acupuncture in rats. Microarray analysis was performed to compare the miRNA expression profiles of medulla in spontaneously hypertensive rats (SHRs) treated with or without acupuncture. Our microarray analysis identified 222 differentially expressed miRNAs in the medulla of SHRs treated with acupuncture at taichong acupoint. Among these miRNAs, 23 miRNAs with a significant difference were found in acupuncture-treated SHRs compared to untreated rats. These 23 miRNAs could regulate 2963 target genes which were enriched in at least 14 pathways based on our bioinformatic analysis. miRNA-339, miR-223, and miR-145 were downregulated in the medulla of SHRs compared to normotensive rats. Notably, these miRNAs were upregulated to basal levels in the medulla of SHRs treated with acupuncture at taichong in comparison with SHRs receiving acupuncture at nonacupoint group or SHRs without any treatment. Our findings have revealed significant changes of a panel of selective miRNAs in hypertensive rats treated at taichong acupoint. These data provide insights into how acupuncture elicits beneficial effects on hypertension.
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spelling pubmed-43774612015-04-08 MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats Wang, Jia-You Li, Hui Ma, Chun-Mei Wang, Jia-Lu Lai, Xin-Sheng Zhou, Shu-Feng Evid Based Complement Alternat Med Research Article MicroRNAs (miRNAs) are a group of endogenous noncoding RNAs that play important roles in many biological processes. This study aimed to check if miRNAs were involved in the response to acupuncture in rats. Microarray analysis was performed to compare the miRNA expression profiles of medulla in spontaneously hypertensive rats (SHRs) treated with or without acupuncture. Our microarray analysis identified 222 differentially expressed miRNAs in the medulla of SHRs treated with acupuncture at taichong acupoint. Among these miRNAs, 23 miRNAs with a significant difference were found in acupuncture-treated SHRs compared to untreated rats. These 23 miRNAs could regulate 2963 target genes which were enriched in at least 14 pathways based on our bioinformatic analysis. miRNA-339, miR-223, and miR-145 were downregulated in the medulla of SHRs compared to normotensive rats. Notably, these miRNAs were upregulated to basal levels in the medulla of SHRs treated with acupuncture at taichong in comparison with SHRs receiving acupuncture at nonacupoint group or SHRs without any treatment. Our findings have revealed significant changes of a panel of selective miRNAs in hypertensive rats treated at taichong acupoint. These data provide insights into how acupuncture elicits beneficial effects on hypertension. Hindawi Publishing Corporation 2015 2015-03-15 /pmc/articles/PMC4377461/ /pubmed/25861353 http://dx.doi.org/10.1155/2015/204367 Text en Copyright © 2015 Jia-You Wang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Jia-You
Li, Hui
Ma, Chun-Mei
Wang, Jia-Lu
Lai, Xin-Sheng
Zhou, Shu-Feng
MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats
title MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats
title_full MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats
title_fullStr MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats
title_full_unstemmed MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats
title_short MicroRNA Profiling Response to Acupuncture Therapy in Spontaneously Hypertensive Rats
title_sort microrna profiling response to acupuncture therapy in spontaneously hypertensive rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377461/
https://www.ncbi.nlm.nih.gov/pubmed/25861353
http://dx.doi.org/10.1155/2015/204367
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