Cargando…

De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification

BACKGROUND: Haloxylon ammodendron (C. A. Mey.) is widely distributed across a range of habitats, including gravel desert, clay desert, fixed and semi-fixed sand, and saline land in Asian and African deserts. To date, no genomic information or expressed sequence tag-simple sequence repeat (EST-SSR) m...

Descripción completa

Detalles Bibliográficos
Autores principales: Long, Yan, Zhang, Jingwen, Tian, Xinjie, Wu, Shanshan, Zhang, Qiong, Zhang, Jianping, Dang, Zhanhai, Pei, Xin Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377846/
https://www.ncbi.nlm.nih.gov/pubmed/25511667
http://dx.doi.org/10.1186/1471-2164-15-1111
_version_ 1782363969853325312
author Long, Yan
Zhang, Jingwen
Tian, Xinjie
Wu, Shanshan
Zhang, Qiong
Zhang, Jianping
Dang, Zhanhai
Pei, Xin Wu
author_facet Long, Yan
Zhang, Jingwen
Tian, Xinjie
Wu, Shanshan
Zhang, Qiong
Zhang, Jianping
Dang, Zhanhai
Pei, Xin Wu
author_sort Long, Yan
collection PubMed
description BACKGROUND: Haloxylon ammodendron (C. A. Mey.) is widely distributed across a range of habitats, including gravel desert, clay desert, fixed and semi-fixed sand, and saline land in Asian and African deserts. To date, no genomic information or expressed sequence tag-simple sequence repeat (EST-SSR) marker has been reported for H. ammodendron plants. RESULTS: Using Illumina sequencing technology, we generated over two billion bases of high-quality sequence data on H. ammodendron and conducted de novo assembly and annotation of genes without prior genome information. These reads were assembled into 79,918 unigenes (mean length = 728 bp). Based on similarity searches comparing these unigenes with known proteins in the non-redundant (nr) protein database, 25,619 unigenes were functionally annotated with a cut-off E-value of 10(-5). In addition, DGE reads were mapped to the assembled transcriptome for gene expression analysis under drought stress. In total, 1,060 differentially expressed genes were identified. Among these genes, 356 genes were upregulated after drought treatment, and 704 genes were downregulated. We used the KEGG database to annotate these drought-induced genes; 207 unigenes were identified in the KEGG pathway annotation, and approximately 12.1% of the unigenes with known function fell into categories related to fatty acid metabolism, starch and sucrose metabolism, and nitrogen metabolism, suggesting that these pathways or processes may be involved in the drought response. Together, a total of 35 drought-inducible transcription factors were identified, including WRKY, MYB and bZIP family members. CONCLUSIONS: Our study is the first to provide a transcriptome sequence resource for H. ammodendron plants and to determine its digital gene expression profile under drought conditions using the assembled transcriptome data for reference. These data provide a valuable resource for genetic and genomic studies of desert plants under abiotic conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-1111) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4377846
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43778462015-03-31 De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification Long, Yan Zhang, Jingwen Tian, Xinjie Wu, Shanshan Zhang, Qiong Zhang, Jianping Dang, Zhanhai Pei, Xin Wu BMC Genomics Research Article BACKGROUND: Haloxylon ammodendron (C. A. Mey.) is widely distributed across a range of habitats, including gravel desert, clay desert, fixed and semi-fixed sand, and saline land in Asian and African deserts. To date, no genomic information or expressed sequence tag-simple sequence repeat (EST-SSR) marker has been reported for H. ammodendron plants. RESULTS: Using Illumina sequencing technology, we generated over two billion bases of high-quality sequence data on H. ammodendron and conducted de novo assembly and annotation of genes without prior genome information. These reads were assembled into 79,918 unigenes (mean length = 728 bp). Based on similarity searches comparing these unigenes with known proteins in the non-redundant (nr) protein database, 25,619 unigenes were functionally annotated with a cut-off E-value of 10(-5). In addition, DGE reads were mapped to the assembled transcriptome for gene expression analysis under drought stress. In total, 1,060 differentially expressed genes were identified. Among these genes, 356 genes were upregulated after drought treatment, and 704 genes were downregulated. We used the KEGG database to annotate these drought-induced genes; 207 unigenes were identified in the KEGG pathway annotation, and approximately 12.1% of the unigenes with known function fell into categories related to fatty acid metabolism, starch and sucrose metabolism, and nitrogen metabolism, suggesting that these pathways or processes may be involved in the drought response. Together, a total of 35 drought-inducible transcription factors were identified, including WRKY, MYB and bZIP family members. CONCLUSIONS: Our study is the first to provide a transcriptome sequence resource for H. ammodendron plants and to determine its digital gene expression profile under drought conditions using the assembled transcriptome data for reference. These data provide a valuable resource for genetic and genomic studies of desert plants under abiotic conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-1111) contains supplementary material, which is available to authorized users. BioMed Central 2014-12-15 /pmc/articles/PMC4377846/ /pubmed/25511667 http://dx.doi.org/10.1186/1471-2164-15-1111 Text en © Long et al.; licensee BioMed Central. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Long, Yan
Zhang, Jingwen
Tian, Xinjie
Wu, Shanshan
Zhang, Qiong
Zhang, Jianping
Dang, Zhanhai
Pei, Xin Wu
De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification
title De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification
title_full De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification
title_fullStr De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification
title_full_unstemmed De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification
title_short De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification
title_sort de novo assembly of the desert tree haloxylon ammodendron (c. a. mey.) based on rna-seq data provides insight into drought response, gene discovery and marker identification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377846/
https://www.ncbi.nlm.nih.gov/pubmed/25511667
http://dx.doi.org/10.1186/1471-2164-15-1111
work_keys_str_mv AT longyan denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT zhangjingwen denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT tianxinjie denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT wushanshan denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT zhangqiong denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT zhangjianping denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT dangzhanhai denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification
AT peixinwu denovoassemblyofthedeserttreehaloxylonammodendroncameybasedonrnaseqdataprovidesinsightintodroughtresponsegenediscoveryandmarkeridentification