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Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway

It is well known that maternal folate deficiency results in adverse pregnancy outcomes. In addition to aspects in embryonic development, maternal uterine receptivity and the decidualization of stromal cells is also very important for a successful pregnancy. In this study, we focused on endometrium d...

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Autores principales: Liao, Xing Gui, Li, Yan Li, Gao, Ru Fei, Geng, Yan Qing, Chen, Xue Mei, Liu, Xue Qing, Ding, Yu Bin, Mu, Xin Yi, Wang, Ying Xiong, He, Jun Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377890/
https://www.ncbi.nlm.nih.gov/pubmed/25781218
http://dx.doi.org/10.3390/nu7031916
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author Liao, Xing Gui
Li, Yan Li
Gao, Ru Fei
Geng, Yan Qing
Chen, Xue Mei
Liu, Xue Qing
Ding, Yu Bin
Mu, Xin Yi
Wang, Ying Xiong
He, Jun Lin
author_facet Liao, Xing Gui
Li, Yan Li
Gao, Ru Fei
Geng, Yan Qing
Chen, Xue Mei
Liu, Xue Qing
Ding, Yu Bin
Mu, Xin Yi
Wang, Ying Xiong
He, Jun Lin
author_sort Liao, Xing Gui
collection PubMed
description It is well known that maternal folate deficiency results in adverse pregnancy outcomes. In addition to aspects in embryonic development, maternal uterine receptivity and the decidualization of stromal cells is also very important for a successful pregnancy. In this study, we focused on endometrium decidualization and investigated whether apoptosis, which is essential for decidualization, was impaired. Flow cytometry and TUNEL detection revealed that apoptosis of mouse endometrium decidual cells was suppressed in the dietary folate-deficient group on Days 7 and 8 of pregnancy (Day 1 = vaginal plug) when decidua regression is initiated. The endometrium decidual tissue of the folate deficiency group expressed less Bax compared to the normal diet group while they had nearly equal expression of Bcl2 protein. Further examination revealed that the mitochondrial transmembrane potential (ΔΨm) decreased, and the fluorescence of diffuse cytoplasmic cytochrome c protein was detected using laser confocal microscopy in normal decidual cells. However, no corresponding changes were observed in the folate-deficient group. Western blotting analyses confirmed that more cytochrome c was released from mitochondria in normal decidual cells. Taken together, these results demonstrated that folate deficiency could inhibit apoptosis of decidual cells via the mitochondrial apoptosis pathway, thereby restraining decidualization of the endometrium and further impairing pregnancy.
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spelling pubmed-43778902015-04-30 Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway Liao, Xing Gui Li, Yan Li Gao, Ru Fei Geng, Yan Qing Chen, Xue Mei Liu, Xue Qing Ding, Yu Bin Mu, Xin Yi Wang, Ying Xiong He, Jun Lin Nutrients Article It is well known that maternal folate deficiency results in adverse pregnancy outcomes. In addition to aspects in embryonic development, maternal uterine receptivity and the decidualization of stromal cells is also very important for a successful pregnancy. In this study, we focused on endometrium decidualization and investigated whether apoptosis, which is essential for decidualization, was impaired. Flow cytometry and TUNEL detection revealed that apoptosis of mouse endometrium decidual cells was suppressed in the dietary folate-deficient group on Days 7 and 8 of pregnancy (Day 1 = vaginal plug) when decidua regression is initiated. The endometrium decidual tissue of the folate deficiency group expressed less Bax compared to the normal diet group while they had nearly equal expression of Bcl2 protein. Further examination revealed that the mitochondrial transmembrane potential (ΔΨm) decreased, and the fluorescence of diffuse cytoplasmic cytochrome c protein was detected using laser confocal microscopy in normal decidual cells. However, no corresponding changes were observed in the folate-deficient group. Western blotting analyses confirmed that more cytochrome c was released from mitochondria in normal decidual cells. Taken together, these results demonstrated that folate deficiency could inhibit apoptosis of decidual cells via the mitochondrial apoptosis pathway, thereby restraining decidualization of the endometrium and further impairing pregnancy. MDPI 2015-03-13 /pmc/articles/PMC4377890/ /pubmed/25781218 http://dx.doi.org/10.3390/nu7031916 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liao, Xing Gui
Li, Yan Li
Gao, Ru Fei
Geng, Yan Qing
Chen, Xue Mei
Liu, Xue Qing
Ding, Yu Bin
Mu, Xin Yi
Wang, Ying Xiong
He, Jun Lin
Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway
title Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway
title_full Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway
title_fullStr Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway
title_full_unstemmed Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway
title_short Folate Deficiency Decreases Apoptosis of Endometrium Decidual Cells in Pregnant Mice via the Mitochondrial Pathway
title_sort folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377890/
https://www.ncbi.nlm.nih.gov/pubmed/25781218
http://dx.doi.org/10.3390/nu7031916
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