Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa

A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4....

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Autores principales: Lv, Shuangshuang, Gao, Jingjing, Liu, Ting, Zhu, Jianhua, Xu, Jian, Song, Liyan, Liang, Jincai, Yu, Rongmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377994/
https://www.ncbi.nlm.nih.gov/pubmed/25789603
http://dx.doi.org/10.3390/md13031466
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author Lv, Shuangshuang
Gao, Jingjing
Liu, Ting
Zhu, Jianhua
Xu, Jian
Song, Liyan
Liang, Jincai
Yu, Rongmin
author_facet Lv, Shuangshuang
Gao, Jingjing
Liu, Ting
Zhu, Jianhua
Xu, Jian
Song, Liyan
Liang, Jincai
Yu, Rongmin
author_sort Lv, Shuangshuang
collection PubMed
description A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing.
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spelling pubmed-43779942015-04-27 Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa Lv, Shuangshuang Gao, Jingjing Liu, Ting Zhu, Jianhua Xu, Jian Song, Liyan Liang, Jincai Yu, Rongmin Mar Drugs Article A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing. MDPI 2015-03-17 /pmc/articles/PMC4377994/ /pubmed/25789603 http://dx.doi.org/10.3390/md13031466 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lv, Shuangshuang
Gao, Jingjing
Liu, Ting
Zhu, Jianhua
Xu, Jian
Song, Liyan
Liang, Jincai
Yu, Rongmin
Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
title Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
title_full Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
title_fullStr Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
title_full_unstemmed Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
title_short Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
title_sort purification and partial characterization of a new antitumor protein from tegillarca granosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377994/
https://www.ncbi.nlm.nih.gov/pubmed/25789603
http://dx.doi.org/10.3390/md13031466
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