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Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex
ATP-dependent chromatin remodeling complexes alter chromatin structure through interactions with chromatin substrates such as DNA, histones, and nucleosomes. However, whether chromatin remodeling complexes have the ability to regulate nonchromatin substrates remains unclear. Saccharomyces cerevisiae...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378192/ https://www.ncbi.nlm.nih.gov/pubmed/25792597 http://dx.doi.org/10.1101/gad.257626.114 |
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author | Kapoor, Prabodh Bao, Yunhe Xiao, Jing Luo, Jie Shen, Jianfeng Persinger, Jim Peng, Guang Ranish, Jeff Bartholomew, Blaine Shen, Xuetong |
author_facet | Kapoor, Prabodh Bao, Yunhe Xiao, Jing Luo, Jie Shen, Jianfeng Persinger, Jim Peng, Guang Ranish, Jeff Bartholomew, Blaine Shen, Xuetong |
author_sort | Kapoor, Prabodh |
collection | PubMed |
description | ATP-dependent chromatin remodeling complexes alter chromatin structure through interactions with chromatin substrates such as DNA, histones, and nucleosomes. However, whether chromatin remodeling complexes have the ability to regulate nonchromatin substrates remains unclear. Saccharomyces cerevisiae checkpoint kinase Mec1 (ATR in mammals) is an essential master regulator of genomic integrity. Here we found that the SWI/SNF chromatin remodeling complex is capable of regulating Mec1 kinase activity. In vivo, Mec1 activity is reduced by the deletion of Snf2, the core ATPase subunit of the SWI/SNF complex. SWI/SNF interacts with Mec1, and cross-linking studies revealed that the Snf2 ATPase is the main interaction partner for Mec1. In vitro, SWI/SNF can activate Mec1 kinase activity in the absence of chromatin or known activators such as Dpb11. The subunit requirement of SWI/SNF-mediated Mec1 regulation differs from that of SWI/SNF-mediated chromatin remodeling. Functionally, SWI/SNF-mediated Mec1 regulation specifically occurs in S phase of the cell cycle. Together, these findings identify a novel regulator of Mec1 kinase activity and suggest that ATP-dependent chromatin remodeling complexes can regulate nonchromatin substrates such as a checkpoint kinase. |
format | Online Article Text |
id | pubmed-4378192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43781922015-09-15 Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex Kapoor, Prabodh Bao, Yunhe Xiao, Jing Luo, Jie Shen, Jianfeng Persinger, Jim Peng, Guang Ranish, Jeff Bartholomew, Blaine Shen, Xuetong Genes Dev Research Papers ATP-dependent chromatin remodeling complexes alter chromatin structure through interactions with chromatin substrates such as DNA, histones, and nucleosomes. However, whether chromatin remodeling complexes have the ability to regulate nonchromatin substrates remains unclear. Saccharomyces cerevisiae checkpoint kinase Mec1 (ATR in mammals) is an essential master regulator of genomic integrity. Here we found that the SWI/SNF chromatin remodeling complex is capable of regulating Mec1 kinase activity. In vivo, Mec1 activity is reduced by the deletion of Snf2, the core ATPase subunit of the SWI/SNF complex. SWI/SNF interacts with Mec1, and cross-linking studies revealed that the Snf2 ATPase is the main interaction partner for Mec1. In vitro, SWI/SNF can activate Mec1 kinase activity in the absence of chromatin or known activators such as Dpb11. The subunit requirement of SWI/SNF-mediated Mec1 regulation differs from that of SWI/SNF-mediated chromatin remodeling. Functionally, SWI/SNF-mediated Mec1 regulation specifically occurs in S phase of the cell cycle. Together, these findings identify a novel regulator of Mec1 kinase activity and suggest that ATP-dependent chromatin remodeling complexes can regulate nonchromatin substrates such as a checkpoint kinase. Cold Spring Harbor Laboratory Press 2015-03-15 /pmc/articles/PMC4378192/ /pubmed/25792597 http://dx.doi.org/10.1101/gad.257626.114 Text en © 2015 Kapoor et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Papers Kapoor, Prabodh Bao, Yunhe Xiao, Jing Luo, Jie Shen, Jianfeng Persinger, Jim Peng, Guang Ranish, Jeff Bartholomew, Blaine Shen, Xuetong Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex |
title | Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex |
title_full | Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex |
title_fullStr | Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex |
title_full_unstemmed | Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex |
title_short | Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex |
title_sort | regulation of mec1 kinase activity by the swi/snf chromatin remodeling complex |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378192/ https://www.ncbi.nlm.nih.gov/pubmed/25792597 http://dx.doi.org/10.1101/gad.257626.114 |
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