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Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae

BACKGROUND: The two-spotted spider mite, Tetranychus urticae, is infected with Wolbachia, which have the ability to manipulate host reproduction and fitness. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in many biological processes such as development, reproduction and host-pathoge...

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Autores principales: Rong, Xia, Zhang, Yan-Kai, Zhang, Kai-Jun, Hong, Xiao-Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378230/
https://www.ncbi.nlm.nih.gov/pubmed/25515563
http://dx.doi.org/10.1186/1471-2164-15-1122
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author Rong, Xia
Zhang, Yan-Kai
Zhang, Kai-Jun
Hong, Xiao-Yue
author_facet Rong, Xia
Zhang, Yan-Kai
Zhang, Kai-Jun
Hong, Xiao-Yue
author_sort Rong, Xia
collection PubMed
description BACKGROUND: The two-spotted spider mite, Tetranychus urticae, is infected with Wolbachia, which have the ability to manipulate host reproduction and fitness. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in many biological processes such as development, reproduction and host-pathogen interactions. Although miRNA was observed to involve in Wolbachia-host interactions in the other insect systems, its roles have not been fully deciphered in the two-spotted spider mite. RESULTS: Small RNA libraries of infected and uninfected T. urticae for both sexes (in total four libraries) were constructed. By integrating the mRNA data originated from the same samples, the target genes of the differentially expressed miRNAs were predicted. Then, GO and pathway analyses were performed for the target genes. Comparison of libraries showed that Wolbachia infection significantly regulated 91 miRNAs in females and 20 miRNAs in males, with an overall suppression of miRNAs in Wolbachia-infected libraries. A comparison of the miRNA and mRNA data predicted that the differentially expressed miRNAs negatively regulated 90 mRNAs in females and 9 mRNAs in males. An analysis of target genes showed that Wolbachia-responsive miRNAs regulated genes with function in sphingolipid metabolism, lysosome function, apoptosis and lipid transporting in both sexes, as well as reproduction in females. CONCLUSION: Comparisons of the miRNA and mRNA data can help to identify miRNAs and miRNA target genes involving in Wolbachia-host interactions. The molecular targets identified in this study should be useful in further functional studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-1122) contains supplementary material, which is available to authorized users.
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spelling pubmed-43782302015-03-31 Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae Rong, Xia Zhang, Yan-Kai Zhang, Kai-Jun Hong, Xiao-Yue BMC Genomics Research Article BACKGROUND: The two-spotted spider mite, Tetranychus urticae, is infected with Wolbachia, which have the ability to manipulate host reproduction and fitness. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in many biological processes such as development, reproduction and host-pathogen interactions. Although miRNA was observed to involve in Wolbachia-host interactions in the other insect systems, its roles have not been fully deciphered in the two-spotted spider mite. RESULTS: Small RNA libraries of infected and uninfected T. urticae for both sexes (in total four libraries) were constructed. By integrating the mRNA data originated from the same samples, the target genes of the differentially expressed miRNAs were predicted. Then, GO and pathway analyses were performed for the target genes. Comparison of libraries showed that Wolbachia infection significantly regulated 91 miRNAs in females and 20 miRNAs in males, with an overall suppression of miRNAs in Wolbachia-infected libraries. A comparison of the miRNA and mRNA data predicted that the differentially expressed miRNAs negatively regulated 90 mRNAs in females and 9 mRNAs in males. An analysis of target genes showed that Wolbachia-responsive miRNAs regulated genes with function in sphingolipid metabolism, lysosome function, apoptosis and lipid transporting in both sexes, as well as reproduction in females. CONCLUSION: Comparisons of the miRNA and mRNA data can help to identify miRNAs and miRNA target genes involving in Wolbachia-host interactions. The molecular targets identified in this study should be useful in further functional studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-1122) contains supplementary material, which is available to authorized users. BioMed Central 2014-12-16 /pmc/articles/PMC4378230/ /pubmed/25515563 http://dx.doi.org/10.1186/1471-2164-15-1122 Text en © Rong et al.; licensee BioMed Central. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Rong, Xia
Zhang, Yan-Kai
Zhang, Kai-Jun
Hong, Xiao-Yue
Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae
title Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae
title_full Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae
title_fullStr Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae
title_full_unstemmed Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae
title_short Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae
title_sort identification of wolbachia-responsive micrornas in the two-spotted spider mite, tetranychus urticae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378230/
https://www.ncbi.nlm.nih.gov/pubmed/25515563
http://dx.doi.org/10.1186/1471-2164-15-1122
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