Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth

Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to...

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Detalles Bibliográficos
Autores principales: Zhu, Hailin, Yang, Jinliang, Wei, Yuquan, Chen, Harry Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378706/
https://www.ncbi.nlm.nih.gov/pubmed/25861280
http://dx.doi.org/10.1155/2015/621057
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author Zhu, Hailin
Yang, Jinliang
Wei, Yuquan
Chen, Harry Huimin
author_facet Zhu, Hailin
Yang, Jinliang
Wei, Yuquan
Chen, Harry Huimin
author_sort Zhu, Hailin
collection PubMed
description Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4°C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.
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spelling pubmed-43787062015-04-08 Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth Zhu, Hailin Yang, Jinliang Wei, Yuquan Chen, Harry Huimin Stem Cells Int Research Article Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4°C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications. Hindawi Publishing Corporation 2015 2015-03-16 /pmc/articles/PMC4378706/ /pubmed/25861280 http://dx.doi.org/10.1155/2015/621057 Text en Copyright © 2015 Hailin Zhu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhu, Hailin
Yang, Jinliang
Wei, Yuquan
Chen, Harry Huimin
Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth
title Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth
title_full Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth
title_fullStr Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth
title_full_unstemmed Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth
title_short Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth
title_sort development of a xeno-free substrate for human embryonic stem cell growth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378706/
https://www.ncbi.nlm.nih.gov/pubmed/25861280
http://dx.doi.org/10.1155/2015/621057
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