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Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins

In Drosophila photoreceptors, the NINAC-encoded myosin III is found in a complex with a small, MORN-repeat containing, protein Retinophilin (RTP). Expression of these two proteins in other cell types showed NINAC myosin III behavior is altered by RTP. NINAC deletion constructs were used to map the R...

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Autores principales: Mecklenburg, Kirk L., Freed, Stephanie A., Raval, Manmeet, Quintero, Omar A., Yengo, Christopher M., O'Tousa, Joseph. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379085/
https://www.ncbi.nlm.nih.gov/pubmed/25822849
http://dx.doi.org/10.1371/journal.pone.0122502
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author Mecklenburg, Kirk L.
Freed, Stephanie A.
Raval, Manmeet
Quintero, Omar A.
Yengo, Christopher M.
O'Tousa, Joseph. E.
author_facet Mecklenburg, Kirk L.
Freed, Stephanie A.
Raval, Manmeet
Quintero, Omar A.
Yengo, Christopher M.
O'Tousa, Joseph. E.
author_sort Mecklenburg, Kirk L.
collection PubMed
description In Drosophila photoreceptors, the NINAC-encoded myosin III is found in a complex with a small, MORN-repeat containing, protein Retinophilin (RTP). Expression of these two proteins in other cell types showed NINAC myosin III behavior is altered by RTP. NINAC deletion constructs were used to map the RTP binding site within the proximal tail domain of NINAC. In vertebrates, the RTP ortholog is MORN4. Co-precipitation experiments demonstrated that human MORN4 binds to human myosin IIIA (MYO3A). In COS7 cells, MORN4 and MYO3A, but not MORN4 and MYO3B, co-localize to actin rich filopodia extensions. Deletion analysis mapped the MORN4 binding to the proximal region of the MYO3A tail domain. MYO3A dependent MORN4 tip localization suggests that MYO3A functions as a motor that transports MORN4 to the filopodia tips and MORN4 may enhance MYO3A tip localization by tethering it to the plasma membrane at the protrusion tips. These results establish conserved features of the RTP/MORN4 family: they bind within the tail domain of myosin IIIs to control their behavior.
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spelling pubmed-43790852015-04-09 Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins Mecklenburg, Kirk L. Freed, Stephanie A. Raval, Manmeet Quintero, Omar A. Yengo, Christopher M. O'Tousa, Joseph. E. PLoS One Research Article In Drosophila photoreceptors, the NINAC-encoded myosin III is found in a complex with a small, MORN-repeat containing, protein Retinophilin (RTP). Expression of these two proteins in other cell types showed NINAC myosin III behavior is altered by RTP. NINAC deletion constructs were used to map the RTP binding site within the proximal tail domain of NINAC. In vertebrates, the RTP ortholog is MORN4. Co-precipitation experiments demonstrated that human MORN4 binds to human myosin IIIA (MYO3A). In COS7 cells, MORN4 and MYO3A, but not MORN4 and MYO3B, co-localize to actin rich filopodia extensions. Deletion analysis mapped the MORN4 binding to the proximal region of the MYO3A tail domain. MYO3A dependent MORN4 tip localization suggests that MYO3A functions as a motor that transports MORN4 to the filopodia tips and MORN4 may enhance MYO3A tip localization by tethering it to the plasma membrane at the protrusion tips. These results establish conserved features of the RTP/MORN4 family: they bind within the tail domain of myosin IIIs to control their behavior. Public Library of Science 2015-03-30 /pmc/articles/PMC4379085/ /pubmed/25822849 http://dx.doi.org/10.1371/journal.pone.0122502 Text en © 2015 Mecklenburg et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mecklenburg, Kirk L.
Freed, Stephanie A.
Raval, Manmeet
Quintero, Omar A.
Yengo, Christopher M.
O'Tousa, Joseph. E.
Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins
title Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins
title_full Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins
title_fullStr Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins
title_full_unstemmed Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins
title_short Invertebrate and Vertebrate Class III Myosins Interact with MORN Repeat-Containing Adaptor Proteins
title_sort invertebrate and vertebrate class iii myosins interact with morn repeat-containing adaptor proteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379085/
https://www.ncbi.nlm.nih.gov/pubmed/25822849
http://dx.doi.org/10.1371/journal.pone.0122502
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