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Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)

A high-brilliance synchrotron P12 beamline of the EMBL located at the PETRA III storage ring (DESY, Hamburg) is dedicated to biological small-angle X-ray scattering (SAXS) and has been designed and optimized for scattering experiments on macromolecular solutions. Scatterless slits reduce the parasit...

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Autores principales: Blanchet, Clement E., Spilotros, Alessandro, Schwemmer, Frank, Graewert, Melissa A., Kikhney, Alexey, Jeffries, Cy M., Franke, Daniel, Mark, Daniel, Zengerle, Roland, Cipriani, Florent, Fiedler, Stefan, Roessle, Manfred, Svergun, Dmitri I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379436/
https://www.ncbi.nlm.nih.gov/pubmed/25844078
http://dx.doi.org/10.1107/S160057671500254X
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author Blanchet, Clement E.
Spilotros, Alessandro
Schwemmer, Frank
Graewert, Melissa A.
Kikhney, Alexey
Jeffries, Cy M.
Franke, Daniel
Mark, Daniel
Zengerle, Roland
Cipriani, Florent
Fiedler, Stefan
Roessle, Manfred
Svergun, Dmitri I.
author_facet Blanchet, Clement E.
Spilotros, Alessandro
Schwemmer, Frank
Graewert, Melissa A.
Kikhney, Alexey
Jeffries, Cy M.
Franke, Daniel
Mark, Daniel
Zengerle, Roland
Cipriani, Florent
Fiedler, Stefan
Roessle, Manfred
Svergun, Dmitri I.
author_sort Blanchet, Clement E.
collection PubMed
description A high-brilliance synchrotron P12 beamline of the EMBL located at the PETRA III storage ring (DESY, Hamburg) is dedicated to biological small-angle X-ray scattering (SAXS) and has been designed and optimized for scattering experiments on macromolecular solutions. Scatterless slits reduce the parasitic scattering, a custom-designed miniature active beamstop ensures accurate data normalization and the photon-counting PILATUS 2M detector enables the background-free detection of weak scattering signals. The high flux and small beam size allow for rapid experiments with exposure time down to 30–50 ms covering the resolution range from about 300 to 0.5 nm. P12 possesses a versatile and flexible sample environment system that caters for the diverse experimental needs required to study macromolecular solutions. These include an in-vacuum capillary mode for standard batch sample analyses with robotic sample delivery and for continuous-flow in-line sample purification and characterization, as well as an in-air capillary time-resolved stopped-flow setup. A novel microfluidic centrifugal mixing device (SAXS disc) is developed for a high-throughput screening mode using sub-microlitre sample volumes. Automation is a key feature of P12; it is controlled by a beamline meta server, which coordinates and schedules experiments from either standard or nonstandard operational setups. The integrated SASFLOW pipeline automatically checks for consistency, and processes and analyses the data, providing near real-time assessments of overall parameters and the generation of low-resolution models within minutes of data collection. These advances, combined with a remote access option, allow for rapid high-throughput analysis, as well as time-resolved and screening experiments for novice and expert biological SAXS users.
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spelling pubmed-43794362015-04-03 Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY) Blanchet, Clement E. Spilotros, Alessandro Schwemmer, Frank Graewert, Melissa A. Kikhney, Alexey Jeffries, Cy M. Franke, Daniel Mark, Daniel Zengerle, Roland Cipriani, Florent Fiedler, Stefan Roessle, Manfred Svergun, Dmitri I. J Appl Crystallogr Research Papers A high-brilliance synchrotron P12 beamline of the EMBL located at the PETRA III storage ring (DESY, Hamburg) is dedicated to biological small-angle X-ray scattering (SAXS) and has been designed and optimized for scattering experiments on macromolecular solutions. Scatterless slits reduce the parasitic scattering, a custom-designed miniature active beamstop ensures accurate data normalization and the photon-counting PILATUS 2M detector enables the background-free detection of weak scattering signals. The high flux and small beam size allow for rapid experiments with exposure time down to 30–50 ms covering the resolution range from about 300 to 0.5 nm. P12 possesses a versatile and flexible sample environment system that caters for the diverse experimental needs required to study macromolecular solutions. These include an in-vacuum capillary mode for standard batch sample analyses with robotic sample delivery and for continuous-flow in-line sample purification and characterization, as well as an in-air capillary time-resolved stopped-flow setup. A novel microfluidic centrifugal mixing device (SAXS disc) is developed for a high-throughput screening mode using sub-microlitre sample volumes. Automation is a key feature of P12; it is controlled by a beamline meta server, which coordinates and schedules experiments from either standard or nonstandard operational setups. The integrated SASFLOW pipeline automatically checks for consistency, and processes and analyses the data, providing near real-time assessments of overall parameters and the generation of low-resolution models within minutes of data collection. These advances, combined with a remote access option, allow for rapid high-throughput analysis, as well as time-resolved and screening experiments for novice and expert biological SAXS users. International Union of Crystallography 2015-03-12 /pmc/articles/PMC4379436/ /pubmed/25844078 http://dx.doi.org/10.1107/S160057671500254X Text en © Clement E. Blanchet et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Blanchet, Clement E.
Spilotros, Alessandro
Schwemmer, Frank
Graewert, Melissa A.
Kikhney, Alexey
Jeffries, Cy M.
Franke, Daniel
Mark, Daniel
Zengerle, Roland
Cipriani, Florent
Fiedler, Stefan
Roessle, Manfred
Svergun, Dmitri I.
Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)
title Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)
title_full Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)
title_fullStr Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)
title_full_unstemmed Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)
title_short Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY)
title_sort versatile sample environments and automation for biological solution x-ray scattering experiments at the p12 beamline (petra iii, desy)
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379436/
https://www.ncbi.nlm.nih.gov/pubmed/25844078
http://dx.doi.org/10.1107/S160057671500254X
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