CaRuby-Nano: a novel high affinity calcium probe for dual color imaging

The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an A...

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Detalles Bibliográficos
Autores principales: Collot, Mayeul, Wilms, Christian D, Bentkhayet, Asma, Marcaggi, Païkan, Couchman, Kiri, Charpak, Serge, Dieudonné, Stéphane, Häusser, Michael, Feltz, Anne, Mallet, Jean-Maurice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379494/
https://www.ncbi.nlm.nih.gov/pubmed/25824291
http://dx.doi.org/10.7554/eLife.05808
Descripción
Sumario:The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments. DOI: http://dx.doi.org/10.7554/eLife.05808.001

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