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CaRuby-Nano: a novel high affinity calcium probe for dual color imaging
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an A...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
eLife Sciences Publications, Ltd
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379494/ https://www.ncbi.nlm.nih.gov/pubmed/25824291 http://dx.doi.org/10.7554/eLife.05808 |
| _version_ | 1782364191696355328 |
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| author | Collot, Mayeul Wilms, Christian D Bentkhayet, Asma Marcaggi, Païkan Couchman, Kiri Charpak, Serge Dieudonné, Stéphane Häusser, Michael Feltz, Anne Mallet, Jean-Maurice |
| author_facet | Collot, Mayeul Wilms, Christian D Bentkhayet, Asma Marcaggi, Païkan Couchman, Kiri Charpak, Serge Dieudonné, Stéphane Häusser, Michael Feltz, Anne Mallet, Jean-Maurice |
| author_sort | Collot, Mayeul |
| collection | PubMed |
| description | The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments. DOI: http://dx.doi.org/10.7554/eLife.05808.001 |
| format | Online Article Text |
| id | pubmed-4379494 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-43794942015-04-02 CaRuby-Nano: a novel high affinity calcium probe for dual color imaging Collot, Mayeul Wilms, Christian D Bentkhayet, Asma Marcaggi, Païkan Couchman, Kiri Charpak, Serge Dieudonné, Stéphane Häusser, Michael Feltz, Anne Mallet, Jean-Maurice eLife Cell Biology The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments. DOI: http://dx.doi.org/10.7554/eLife.05808.001 eLife Sciences Publications, Ltd 2015-03-31 /pmc/articles/PMC4379494/ /pubmed/25824291 http://dx.doi.org/10.7554/eLife.05808 Text en © 2015, Collot et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
| spellingShingle | Cell Biology Collot, Mayeul Wilms, Christian D Bentkhayet, Asma Marcaggi, Païkan Couchman, Kiri Charpak, Serge Dieudonné, Stéphane Häusser, Michael Feltz, Anne Mallet, Jean-Maurice CaRuby-Nano: a novel high affinity calcium probe for dual color imaging |
| title | CaRuby-Nano: a novel high affinity calcium probe for dual color imaging |
| title_full | CaRuby-Nano: a novel high affinity calcium probe for dual color imaging |
| title_fullStr | CaRuby-Nano: a novel high affinity calcium probe for dual color imaging |
| title_full_unstemmed | CaRuby-Nano: a novel high affinity calcium probe for dual color imaging |
| title_short | CaRuby-Nano: a novel high affinity calcium probe for dual color imaging |
| title_sort | caruby-nano: a novel high affinity calcium probe for dual color imaging |
| topic | Cell Biology |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379494/ https://www.ncbi.nlm.nih.gov/pubmed/25824291 http://dx.doi.org/10.7554/eLife.05808 |
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