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Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study

A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid...

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Autores principales: Rodríguez-Carrasco, Yelko, Mañes, Jordi, Berrada, Houda, Font, Guillermina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379519/
https://www.ncbi.nlm.nih.gov/pubmed/25723325
http://dx.doi.org/10.3390/toxins7030705
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author Rodríguez-Carrasco, Yelko
Mañes, Jordi
Berrada, Houda
Font, Guillermina
author_facet Rodríguez-Carrasco, Yelko
Mañes, Jordi
Berrada, Houda
Font, Guillermina
author_sort Rodríguez-Carrasco, Yelko
collection PubMed
description A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method’s accuracy was in a range of 68%–108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.
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spelling pubmed-43795192015-05-18 Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study Rodríguez-Carrasco, Yelko Mañes, Jordi Berrada, Houda Font, Guillermina Toxins (Basel) Article A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method’s accuracy was in a range of 68%–108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies. MDPI 2015-02-25 /pmc/articles/PMC4379519/ /pubmed/25723325 http://dx.doi.org/10.3390/toxins7030705 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Carrasco, Yelko
Mañes, Jordi
Berrada, Houda
Font, Guillermina
Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
title Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
title_full Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
title_fullStr Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
title_full_unstemmed Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
title_short Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
title_sort preliminary estimation of deoxynivalenol excretion through a 24 h pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379519/
https://www.ncbi.nlm.nih.gov/pubmed/25723325
http://dx.doi.org/10.3390/toxins7030705
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