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Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study
A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379519/ https://www.ncbi.nlm.nih.gov/pubmed/25723325 http://dx.doi.org/10.3390/toxins7030705 |
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author | Rodríguez-Carrasco, Yelko Mañes, Jordi Berrada, Houda Font, Guillermina |
author_facet | Rodríguez-Carrasco, Yelko Mañes, Jordi Berrada, Houda Font, Guillermina |
author_sort | Rodríguez-Carrasco, Yelko |
collection | PubMed |
description | A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method’s accuracy was in a range of 68%–108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies. |
format | Online Article Text |
id | pubmed-4379519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43795192015-05-18 Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study Rodríguez-Carrasco, Yelko Mañes, Jordi Berrada, Houda Font, Guillermina Toxins (Basel) Article A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method’s accuracy was in a range of 68%–108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies. MDPI 2015-02-25 /pmc/articles/PMC4379519/ /pubmed/25723325 http://dx.doi.org/10.3390/toxins7030705 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rodríguez-Carrasco, Yelko Mañes, Jordi Berrada, Houda Font, Guillermina Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study |
title | Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study |
title_full | Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study |
title_fullStr | Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study |
title_full_unstemmed | Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study |
title_short | Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study |
title_sort | preliminary estimation of deoxynivalenol excretion through a 24 h pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379519/ https://www.ncbi.nlm.nih.gov/pubmed/25723325 http://dx.doi.org/10.3390/toxins7030705 |
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