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Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China

Infection of poultry with diverse lineages of H5N2 avian influenza viruses has been documented for over three decades in different parts of the world, with limited outbreaks caused by this highly pathogenic avian influenza virus. In the present study, three avian H5N2 influenza viruses, A/chicken/Sh...

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Autores principales: Xu, Huaiying, Meng, Fang, Huang, Dihai, Sheng, Xiaodan, Wang, Youling, Zhang, Wei, Chang, Weishan, Wang, Leyi, Qin, Zhuoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379553/
https://www.ncbi.nlm.nih.gov/pubmed/25723387
http://dx.doi.org/10.3390/v7030887
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author Xu, Huaiying
Meng, Fang
Huang, Dihai
Sheng, Xiaodan
Wang, Youling
Zhang, Wei
Chang, Weishan
Wang, Leyi
Qin, Zhuoming
author_facet Xu, Huaiying
Meng, Fang
Huang, Dihai
Sheng, Xiaodan
Wang, Youling
Zhang, Wei
Chang, Weishan
Wang, Leyi
Qin, Zhuoming
author_sort Xu, Huaiying
collection PubMed
description Infection of poultry with diverse lineages of H5N2 avian influenza viruses has been documented for over three decades in different parts of the world, with limited outbreaks caused by this highly pathogenic avian influenza virus. In the present study, three avian H5N2 influenza viruses, A/chicken/Shijiazhuang/1209/2013, A/chicken/Chiping/0321/2014, and A/chicken/Laiwu/0313/2014, were isolated from chickens with clinical symptoms of avian influenza. Complete genomic and phylogenetic analyses demonstrated that all three isolates are novel recombinant viruses with hemagglutinin (HA) and matrix (M) genes derived from H5N1, and remaining genes derived from H9N2-like viruses. The HA cleavage motif in all three strains (PQIEGRRRKR/GL) is characteristic of a highly pathogenic avian influenza virus strain. These results indicate the occurrence of H5N2 recombination and highlight the importance of continued surveillance of the H5N2 subtype virus and reformulation of vaccine strains.
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spelling pubmed-43795532015-05-05 Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China Xu, Huaiying Meng, Fang Huang, Dihai Sheng, Xiaodan Wang, Youling Zhang, Wei Chang, Weishan Wang, Leyi Qin, Zhuoming Viruses Article Infection of poultry with diverse lineages of H5N2 avian influenza viruses has been documented for over three decades in different parts of the world, with limited outbreaks caused by this highly pathogenic avian influenza virus. In the present study, three avian H5N2 influenza viruses, A/chicken/Shijiazhuang/1209/2013, A/chicken/Chiping/0321/2014, and A/chicken/Laiwu/0313/2014, were isolated from chickens with clinical symptoms of avian influenza. Complete genomic and phylogenetic analyses demonstrated that all three isolates are novel recombinant viruses with hemagglutinin (HA) and matrix (M) genes derived from H5N1, and remaining genes derived from H9N2-like viruses. The HA cleavage motif in all three strains (PQIEGRRRKR/GL) is characteristic of a highly pathogenic avian influenza virus strain. These results indicate the occurrence of H5N2 recombination and highlight the importance of continued surveillance of the H5N2 subtype virus and reformulation of vaccine strains. MDPI 2015-02-25 /pmc/articles/PMC4379553/ /pubmed/25723387 http://dx.doi.org/10.3390/v7030887 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Huaiying
Meng, Fang
Huang, Dihai
Sheng, Xiaodan
Wang, Youling
Zhang, Wei
Chang, Weishan
Wang, Leyi
Qin, Zhuoming
Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
title Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
title_full Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
title_fullStr Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
title_full_unstemmed Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
title_short Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
title_sort genomic and phylogenetic characterization of novel, recombinant h5n2 avian influenza virus strains isolated from vaccinated chickens with clinical symptoms in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379553/
https://www.ncbi.nlm.nih.gov/pubmed/25723387
http://dx.doi.org/10.3390/v7030887
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