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Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays

Introduction: Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy. Methods: We used a novel proteome microarray technology and statistical modeling to identify factors and...

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Autores principales: Haralambieva, Iana H., Simon, Whitney L., Kennedy, Richard B., Ovsyannikova, Inna G., Warner, Nathaniel D., Grill, Diane E., Poland, Gregory A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379563/
https://www.ncbi.nlm.nih.gov/pubmed/25763865
http://dx.doi.org/10.3390/v7031113
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author Haralambieva, Iana H.
Simon, Whitney L.
Kennedy, Richard B.
Ovsyannikova, Inna G.
Warner, Nathaniel D.
Grill, Diane E.
Poland, Gregory A.
author_facet Haralambieva, Iana H.
Simon, Whitney L.
Kennedy, Richard B.
Ovsyannikova, Inna G.
Warner, Nathaniel D.
Grill, Diane E.
Poland, Gregory A.
author_sort Haralambieva, Iana H.
collection PubMed
description Introduction: Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy. Methods: We used a novel proteome microarray technology and statistical modeling to identify factors and models associated with measles-specific functional protective immunity in 150 measles vaccine recipients representing the extremes of neutralizing antibody response after two vaccine doses. Results: Our findings demonstrate a high seroprevalence of antibodies directed to the measles virus (MV) phosphoprotein (P), nucleoprotein (N), as well as antibodies to the large polymerase (L) protein (fragment 1234 to 1900 AA). Antibodies to these proteins, in addition to anti-F antibodies (and, to a lesser extent, anti-H antibodies), were correlated with neutralizing antibody titer and/or were associated with and predictive of neutralizing antibody response. Conclusion: Our results identify antibodies to specific measles virus proteins and statistical models for monitoring and assessment of measles-specific functional protective immunity in vaccinated individuals.
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spelling pubmed-43795632015-05-05 Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays Haralambieva, Iana H. Simon, Whitney L. Kennedy, Richard B. Ovsyannikova, Inna G. Warner, Nathaniel D. Grill, Diane E. Poland, Gregory A. Viruses Article Introduction: Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy. Methods: We used a novel proteome microarray technology and statistical modeling to identify factors and models associated with measles-specific functional protective immunity in 150 measles vaccine recipients representing the extremes of neutralizing antibody response after two vaccine doses. Results: Our findings demonstrate a high seroprevalence of antibodies directed to the measles virus (MV) phosphoprotein (P), nucleoprotein (N), as well as antibodies to the large polymerase (L) protein (fragment 1234 to 1900 AA). Antibodies to these proteins, in addition to anti-F antibodies (and, to a lesser extent, anti-H antibodies), were correlated with neutralizing antibody titer and/or were associated with and predictive of neutralizing antibody response. Conclusion: Our results identify antibodies to specific measles virus proteins and statistical models for monitoring and assessment of measles-specific functional protective immunity in vaccinated individuals. MDPI 2015-03-10 /pmc/articles/PMC4379563/ /pubmed/25763865 http://dx.doi.org/10.3390/v7031113 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Haralambieva, Iana H.
Simon, Whitney L.
Kennedy, Richard B.
Ovsyannikova, Inna G.
Warner, Nathaniel D.
Grill, Diane E.
Poland, Gregory A.
Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays
title Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays
title_full Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays
title_fullStr Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays
title_full_unstemmed Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays
title_short Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays
title_sort profiling of measles-specific humoral immunity in individuals following two doses of mmr vaccine using proteome microarrays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379563/
https://www.ncbi.nlm.nih.gov/pubmed/25763865
http://dx.doi.org/10.3390/v7031113
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