Cargando…

Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine

BACKGROUND: Attempts to enrich or identify DNA with cytosine methylation have been commonly carried out using anti-5-methylcytosine or anti-MBD2 (methyl-CpG binding domain protein 2) antibody in immunoprecipitation (IP) assays. However, a careful and systematic control experiment to examine the sens...

Descripción completa

Detalles Bibliográficos
Autores principales:	Okitsu, Cindy Y, Hsieh, Chih-Lin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379710/ https://www.ncbi.nlm.nih.gov/pubmed/25888776 http://dx.doi.org/10.1186/s13104-015-1069-0

Ejemplares similares

Reproducibility and reliability of SNP analysis using human cellular DNA at or near nanogram levels
por: Okitsu, Cindy Y, et al.
Publicado: (2013)

5-methylcytosine-sensitive variants of Thermococcus kodakaraensis DNA polymerase
por: Huber, Claudia, et al.
Publicado: (2016)

Assessing the efficiency and significance of Methylated DNA Immunoprecipitation (MeDIP) assays in using in vitro methylated genomic DNA
por: Jia, Jinsong, et al.
Publicado: (2010)

Crystal structures of B-DNA dodecamer containing the epigenetic modifications 5-hydroxymethylcytosine or 5-methylcytosine
por: Renciuk, Daniel, et al.
Publicado: (2013)

Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine
por: Jin, Seung-Gi, et al.
Publicado: (2010)

Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end
por: Kurita, Hisaka, et al.
Publicado: (2018)

Variable presence of 5-methylcytosine in commercial RNA and DNA
por: Schmid, Katharina, et al.
Publicado: (2015)

Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA
por: Matsuda, Shigeru, et al.
Publicado: (2018)

Epigenetics of Modified DNA Bases: 5-Methylcytosine and Beyond
por: Kumar, Suresh, et al.
Publicado: (2018)

Application of 5-Methylcytosine DNA Glycosylase to the Quantitative Analysis of DNA Methylation
por: Choi, Woo Lee, et al.
Publicado: (2021)

Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond
por: Breiling, Achim, et al.
Publicado: (2015)

Placental 5-methylcytosine and 5-hydroxymethylcytosine patterns associate with size at birth
por: Piyasena, Chinthika, et al.
Publicado: (2015)

Enhanced nucleosome assembly at CpG sites containing an extended 5-methylcytosine analogue
por: Tomkuvienė, Miglė, et al.
Publicado: (2022)

Site- and degree-specific C–H oxidation on 5-methylcytosine homologues for probing active DNA demethylation
por: Kavoosi, Sam, et al.
Publicado: (2019)

Recognition and cleavage of 5-methylcytosine DNA by bacterial SRA-HNH proteins
por: Han, Tiesheng, et al.
Publicado: (2015)

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA
por: Gutjahr, Alice, et al.
Publicado: (2014)

Hydroxyl-radical-induced oxidation of 5-methylcytosine in isolated and cellular DNA
por: Madugundu, Guru S., et al.
Publicado: (2014)

Characterizing 5-methylcytosine in the mammalian epitranscriptome
por: Hussain, Shobbir, et al.
Publicado: (2013)

A Mutant of Uracil DNA Glycosylase That Distinguishes between Cytosine and 5-Methylcytosine
por: Kimber, Scott T., et al.
Publicado: (2014)

5-Methylcytosine Recognition by Arabidopsis thaliana DNA Glycosylases DEMETER and DML3
por: Brooks, Sonja C., et al.
Publicado: (2014)

Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA
por: Hashimoto, Hideharu, et al.
Publicado: (2013)

Analysis of 5-Methylcytosine Regulators and DNA Methylation-Driven Genes in Colon Cancer
por: Du, Cheng, et al.
Publicado: (2022)

DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B
por: Fu, Yang, et al.
Publicado: (2015)

Global and Site-Specific Changes in 5-Methylcytosine and 5-Hydroxymethylcytosine after Extended Post-mortem Interval
por: Gross, Jeffrey A., et al.
Publicado: (2016)

Deamination of 5-Methylcytosine Residues in Mammalian Cells
por: Gromenko, E.V., et al.
Publicado: (2009)

Discovery of a Bacterial 5-Methylcytosine Deaminase
por: Hitchcock, Daniel S., et al.
Publicado: (2014)

Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
por: Singh, Aditi, et al.
Publicado: (2018)

Solid-Phase Synthesis of Oligodeoxynucleotides Containing N(4)-[2-(t-butyldisulfanyl)ethyl]-5-methylcytosine Moieties
por: Pérez-Rentero, Sónia, et al.
Publicado: (2010)

A discontinuous DNA glycosylase domain in a family of enzymes that excise 5-methylcytosine
por: Ponferrada-Marín, María Isabel, et al.
Publicado: (2011)

Quantitative assessment of Tet-induced oxidation products of 5-methylcytosine in cellular and tissue DNA
por: Liu, Shuo, et al.
Publicado: (2013)

Effects of Tet-Induced Oxidation Products of 5-Methylcytosine on DNA Replication in Mammalian Cells
por: Ji, Debin, et al.
Publicado: (2014)

Engineering DNA Backbone Interactions Results in TALE Scaffolds with Enhanced 5-Methylcytosine Selectivity
por: Rathi, Preeti, et al.
Publicado: (2017)

DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices
por: Liu, Ming, et al.
Publicado: (2020)

Chemical and enzymatic modifications of 5-methylcytosine at the intersection of DNA damage, repair, and epigenetic reprogramming
por: Baljinnyam, Tuvshintugs, et al.
Publicado: (2022)

Optimized bisulfite sequencing analysis reveals the lack of 5-methylcytosine in mammalian mitochondrial DNA
por: Shao, Zhenyu, et al.
Publicado: (2023)

The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine
por: Sukackaite, Rasa, et al.
Publicado: (2012)

5-Methylcytosine and 5-Hydroxymethylcytosine Spatiotemporal Profiles in the Mouse Zygote
por: Salvaing, Juliette, et al.
Publicado: (2012)

Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
por: Zhang, Yuan, et al.
Publicado: (2017)

5-Methylcytosine and 5-Hydroxymethylcytosine Signatures Underlying Pediatric Cancers
por: Jhanwar, Shalu, et al.
Publicado: (2019)

Association of 5-Methylcytosine and 5-Hydroxymethylcytosine with Mitochondrial DNA Content and Clinical and Biochemical Parameters in Hepatocellular Carcinoma
por: Shen, Fan, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_0ghltt8pg8sj5vk46vgr86rpif