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Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli

BACKGROUND: Human tissue plasminogen activator (tPA) belongs to the serine protease family. It converts plasminogen into plasmin and is used clinically to treat thrombosis. Human tPA is composed of 527 amino acids residues and contains 17 disulfide bonds. Escherichia coli has been used only rarely f...

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Detalles Bibliográficos
Autores principales:	Long, Xiaobin, Gou, Yeran, Luo, Miao, Zhang, Shaocheng, Zhang, Hongpeng, Bai, Lei, Wu, Shuang, He, Quan, Chen, Ke, Huang, Ailong, Zhou, Jianzhong, Wang, Deqiang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379951/ https://www.ncbi.nlm.nih.gov/pubmed/25886739 http://dx.doi.org/10.1186/s12896-015-0127-y

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