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Reference Gene Validation for RT-qPCR, a Note on Different Available Software Packages

BACKGROUND: An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is stably expressed between cell types or...

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Detalles Bibliográficos
Autores principales: De Spiegelaere, Ward, Dern-Wieloch, Jutta, Weigel, Roswitha, Schumacher, Valérie, Schorle, Hubert, Nettersheim, Daniel, Bergmann, Martin, Brehm, Ralph, Kliesch, Sabine, Vandekerckhove, Linos, Fink, Cornelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4380439/
https://www.ncbi.nlm.nih.gov/pubmed/25825906
http://dx.doi.org/10.1371/journal.pone.0122515