Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays

ELISPOT, ELISA and flow cytometry techniques are often used to study the function of immune system cells. It is tempting to speculate that these assays can be used interchangeably, providing similar information about the cytokine secreting activity of cells: the higher the number of cytokine-positiv...

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Detalles Bibliográficos
Autores principales: Hagen, Jodi, Zimmerman, Ryan, Goetz, Christine, Bonnevier, Jody, Houchins, Jeffrey P., Reagan, Kevin, Kalyuzhny, Alexander E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381211/
https://www.ncbi.nlm.nih.gov/pubmed/25679284
http://dx.doi.org/10.3390/cells4010084

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381211/
https://www.ncbi.nlm.nih.gov/pubmed/25679284
http://dx.doi.org/10.3390/cells4010084

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