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Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays
ELISPOT, ELISA and flow cytometry techniques are often used to study the function of immune system cells. It is tempting to speculate that these assays can be used interchangeably, providing similar information about the cytokine secreting activity of cells: the higher the number of cytokine-positiv...
Autores principales: | Hagen, Jodi, Zimmerman, Ryan, Goetz, Christine, Bonnevier, Jody, Houchins, Jeffrey P., Reagan, Kevin, Kalyuzhny, Alexander E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381211/ https://www.ncbi.nlm.nih.gov/pubmed/25679284 http://dx.doi.org/10.3390/cells4010084 |
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