Easy and Rapid Binding Assay for Functional Analysis of Disulfide-Containing Peptides by a Pull-Down Method Using a Puromycin-Linker and a Cell-Free Translation System

Constrained peptides are an attractive class as affinity reagents or drug leads owing to their excellent binding properties. Many kinds of these peptides, such as cyclic peptides containing disulfide bridges, are found in nature or designed artificially by directed evolution. However, confirming the...

Descripción completa

Detalles Bibliográficos
Autores principales: Tanemura, Yutaro, Mochizuki, Yuki, Kumachi, Shigefumi, Nemoto, Naoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381223/
https://www.ncbi.nlm.nih.gov/pubmed/25738808
http://dx.doi.org/10.3390/biology4010161

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381223/
https://www.ncbi.nlm.nih.gov/pubmed/25738808
http://dx.doi.org/10.3390/biology4010161

Ejemplares similares