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A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381293/ https://www.ncbi.nlm.nih.gov/pubmed/25734341 http://dx.doi.org/10.3390/metabo5010119 |
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author | Daskalaki, Evangelia Blackburn, Gavin Kalna, Gabriela Zhang, Tong Anthony, Nahoum Watson, David G. |
author_facet | Daskalaki, Evangelia Blackburn, Gavin Kalna, Gabriela Zhang, Tong Anthony, Nahoum Watson, David G. |
author_sort | Daskalaki, Evangelia |
collection | PubMed |
description | Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h aerobic exercise session in the urine of three physically active males. Individual urine samples were collected over a 37-h protocol (two pre-exercise and eight post-exercise). Raw data were subjected to a variety of normalization techniques, with the most effective measure dividing each metabolite by the sum response of that metabolite for each individual across the 37-h protocol expressed as a percentage. This allowed the metabolite responses to be plotted on a normalised scale. Our results highlight significant metabolites located in the following systems: purine pathway, tryptophan metabolism, carnitine metabolism, cortisol metabolism, androgen metabolism, amino acid oxidation, as well as metabolites from the gastrointestinal microbiome. Many of the significant changes observed in our pilot investigation mirror previous research studies, of various methodological designs, published within the last 15 years, although they have never been reported at the same time in a single study. |
format | Online Article Text |
id | pubmed-4381293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43812932015-05-18 A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output Daskalaki, Evangelia Blackburn, Gavin Kalna, Gabriela Zhang, Tong Anthony, Nahoum Watson, David G. Metabolites Article Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h aerobic exercise session in the urine of three physically active males. Individual urine samples were collected over a 37-h protocol (two pre-exercise and eight post-exercise). Raw data were subjected to a variety of normalization techniques, with the most effective measure dividing each metabolite by the sum response of that metabolite for each individual across the 37-h protocol expressed as a percentage. This allowed the metabolite responses to be plotted on a normalised scale. Our results highlight significant metabolites located in the following systems: purine pathway, tryptophan metabolism, carnitine metabolism, cortisol metabolism, androgen metabolism, amino acid oxidation, as well as metabolites from the gastrointestinal microbiome. Many of the significant changes observed in our pilot investigation mirror previous research studies, of various methodological designs, published within the last 15 years, although they have never been reported at the same time in a single study. MDPI 2015-02-27 /pmc/articles/PMC4381293/ /pubmed/25734341 http://dx.doi.org/10.3390/metabo5010119 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Daskalaki, Evangelia Blackburn, Gavin Kalna, Gabriela Zhang, Tong Anthony, Nahoum Watson, David G. A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output |
title | A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output |
title_full | A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output |
title_fullStr | A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output |
title_full_unstemmed | A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output |
title_short | A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output |
title_sort | study of the effects of exercise on the urinary metabolome using normalisation to individual metabolic output |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381293/ https://www.ncbi.nlm.nih.gov/pubmed/25734341 http://dx.doi.org/10.3390/metabo5010119 |
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