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A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output

Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h...

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Autores principales: Daskalaki, Evangelia, Blackburn, Gavin, Kalna, Gabriela, Zhang, Tong, Anthony, Nahoum, Watson, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381293/
https://www.ncbi.nlm.nih.gov/pubmed/25734341
http://dx.doi.org/10.3390/metabo5010119
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author Daskalaki, Evangelia
Blackburn, Gavin
Kalna, Gabriela
Zhang, Tong
Anthony, Nahoum
Watson, David G.
author_facet Daskalaki, Evangelia
Blackburn, Gavin
Kalna, Gabriela
Zhang, Tong
Anthony, Nahoum
Watson, David G.
author_sort Daskalaki, Evangelia
collection PubMed
description Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h aerobic exercise session in the urine of three physically active males. Individual urine samples were collected over a 37-h protocol (two pre-exercise and eight post-exercise). Raw data were subjected to a variety of normalization techniques, with the most effective measure dividing each metabolite by the sum response of that metabolite for each individual across the 37-h protocol expressed as a percentage. This allowed the metabolite responses to be plotted on a normalised scale. Our results highlight significant metabolites located in the following systems: purine pathway, tryptophan metabolism, carnitine metabolism, cortisol metabolism, androgen metabolism, amino acid oxidation, as well as metabolites from the gastrointestinal microbiome. Many of the significant changes observed in our pilot investigation mirror previous research studies, of various methodological designs, published within the last 15 years, although they have never been reported at the same time in a single study.
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spelling pubmed-43812932015-05-18 A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output Daskalaki, Evangelia Blackburn, Gavin Kalna, Gabriela Zhang, Tong Anthony, Nahoum Watson, David G. Metabolites Article Aerobic exercise, in spite of its multi-organ benefit and potent effect on the metabolome, has yet to be investigated comprehensively via an untargeted metabolomics technology. We conducted an exploratory untargeted liquid chromatography mass spectrometry study to investigate the effects of a one-h aerobic exercise session in the urine of three physically active males. Individual urine samples were collected over a 37-h protocol (two pre-exercise and eight post-exercise). Raw data were subjected to a variety of normalization techniques, with the most effective measure dividing each metabolite by the sum response of that metabolite for each individual across the 37-h protocol expressed as a percentage. This allowed the metabolite responses to be plotted on a normalised scale. Our results highlight significant metabolites located in the following systems: purine pathway, tryptophan metabolism, carnitine metabolism, cortisol metabolism, androgen metabolism, amino acid oxidation, as well as metabolites from the gastrointestinal microbiome. Many of the significant changes observed in our pilot investigation mirror previous research studies, of various methodological designs, published within the last 15 years, although they have never been reported at the same time in a single study. MDPI 2015-02-27 /pmc/articles/PMC4381293/ /pubmed/25734341 http://dx.doi.org/10.3390/metabo5010119 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Daskalaki, Evangelia
Blackburn, Gavin
Kalna, Gabriela
Zhang, Tong
Anthony, Nahoum
Watson, David G.
A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
title A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
title_full A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
title_fullStr A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
title_full_unstemmed A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
title_short A Study of the Effects of Exercise on the Urinary Metabolome Using Normalisation to Individual Metabolic Output
title_sort study of the effects of exercise on the urinary metabolome using normalisation to individual metabolic output
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381293/
https://www.ncbi.nlm.nih.gov/pubmed/25734341
http://dx.doi.org/10.3390/metabo5010119
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