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Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development

BACKGROUND: The effectiveness of bioremediation systems for PAH-contaminated soil may be constrained by physicochemical properties of contaminants and environmental factors. Information on what is the most effective factor in bioremediation process is essential in the decision of what stimulations c...

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Autores principales: Ravanipour, Masoumeh, Kalantary, Roshanak Rezaei, Mohseni-Bandpi, Anoushiravan, Esrafili, Ali, Farzadkia, Mahdi, Hashemi-Najafabadi, Samireh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381363/
https://www.ncbi.nlm.nih.gov/pubmed/25834738
http://dx.doi.org/10.1186/s40201-015-0178-y
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author Ravanipour, Masoumeh
Kalantary, Roshanak Rezaei
Mohseni-Bandpi, Anoushiravan
Esrafili, Ali
Farzadkia, Mahdi
Hashemi-Najafabadi, Samireh
author_facet Ravanipour, Masoumeh
Kalantary, Roshanak Rezaei
Mohseni-Bandpi, Anoushiravan
Esrafili, Ali
Farzadkia, Mahdi
Hashemi-Najafabadi, Samireh
author_sort Ravanipour, Masoumeh
collection PubMed
description BACKGROUND: The effectiveness of bioremediation systems for PAH-contaminated soil may be constrained by physicochemical properties of contaminants and environmental factors. Information on what is the most effective factor in bioremediation process is essential in the decision of what stimulations can be taken to assist the biodegradation efficacy. METHODS: In this study, four factors of surfactant (Tween 80), humic acid (HA), salinity and nutrients in a 2(4) full factorial design were screened in bioremediation of phenanthrene contaminated soil by using a consortium of bacteria. RESULTS: Between the employed levels of the factors only salinity had not significant effect. Optimal concentrations of surfactant, HA and nutrient were obtained by a response surface design. For phenanthrene biodegradation, a central composite face centred design (CCFD) showed that nutrient, surfactant and HA concentrations had highly significant, significant and insignificant effects, respectively. The best conditions with 87.1% phenanthrene biodegradation were 150 mg HA/Kg soil, 12.68 μg/L surfactant, and nutrients as K(2)HPO(4), 0.8; KH(2)PO(4), 0.2 and KNO(3), 1 g/L. A high similarity was between the model prediction and experimental results. CONCLUSIONS: This study showed that nutrient with 81.27% efficiency could be considered as the most effective factor for practical implications of bioremediation process for PAHs contaminated soil cleanup strategies.
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spelling pubmed-43813632015-04-02 Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development Ravanipour, Masoumeh Kalantary, Roshanak Rezaei Mohseni-Bandpi, Anoushiravan Esrafili, Ali Farzadkia, Mahdi Hashemi-Najafabadi, Samireh J Environ Health Sci Eng Research Article BACKGROUND: The effectiveness of bioremediation systems for PAH-contaminated soil may be constrained by physicochemical properties of contaminants and environmental factors. Information on what is the most effective factor in bioremediation process is essential in the decision of what stimulations can be taken to assist the biodegradation efficacy. METHODS: In this study, four factors of surfactant (Tween 80), humic acid (HA), salinity and nutrients in a 2(4) full factorial design were screened in bioremediation of phenanthrene contaminated soil by using a consortium of bacteria. RESULTS: Between the employed levels of the factors only salinity had not significant effect. Optimal concentrations of surfactant, HA and nutrient were obtained by a response surface design. For phenanthrene biodegradation, a central composite face centred design (CCFD) showed that nutrient, surfactant and HA concentrations had highly significant, significant and insignificant effects, respectively. The best conditions with 87.1% phenanthrene biodegradation were 150 mg HA/Kg soil, 12.68 μg/L surfactant, and nutrients as K(2)HPO(4), 0.8; KH(2)PO(4), 0.2 and KNO(3), 1 g/L. A high similarity was between the model prediction and experimental results. CONCLUSIONS: This study showed that nutrient with 81.27% efficiency could be considered as the most effective factor for practical implications of bioremediation process for PAHs contaminated soil cleanup strategies. BioMed Central 2015-03-20 /pmc/articles/PMC4381363/ /pubmed/25834738 http://dx.doi.org/10.1186/s40201-015-0178-y Text en © Ravanipour et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ravanipour, Masoumeh
Kalantary, Roshanak Rezaei
Mohseni-Bandpi, Anoushiravan
Esrafili, Ali
Farzadkia, Mahdi
Hashemi-Najafabadi, Samireh
Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development
title Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development
title_full Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development
title_fullStr Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development
title_full_unstemmed Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development
title_short Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development
title_sort experimental design approach to the optimization of pahs bioremediation from artificially contaminated soil: application of variables screening development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381363/
https://www.ncbi.nlm.nih.gov/pubmed/25834738
http://dx.doi.org/10.1186/s40201-015-0178-y
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