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I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
In both vertebrates and invertebrates, photoreceptors’ output is regulated by feedback signals from interneurons that contribute to several important visual functions. Although synaptic feedback regulation of photoreceptors is known to occur in Drosophila, many questions about the underlying molecul...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382183/ https://www.ncbi.nlm.nih.gov/pubmed/25831426 http://dx.doi.org/10.1371/journal.pbio.1002115 |
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author | Hu, Wen Wang, Tingting Wang, Xiao Han, Junhai |
author_facet | Hu, Wen Wang, Tingting Wang, Xiao Han, Junhai |
author_sort | Hu, Wen |
collection | PubMed |
description | In both vertebrates and invertebrates, photoreceptors’ output is regulated by feedback signals from interneurons that contribute to several important visual functions. Although synaptic feedback regulation of photoreceptors is known to occur in Drosophila, many questions about the underlying molecular mechanisms and physiological implementation remain unclear. Here, we systematically investigated these questions using a broad range of experimental methods. We isolated two I(h) mutant fly lines that exhibit rhythmic photoreceptor depolarization without light stimulation. We discovered that I(h) channels regulate glutamate release from amacrine cells by modulating calcium channel activity. Moreover, we showed that the eye-enriched kainate receptor (EKAR) is expressed in photoreceptors and receives the glutamate signal released from amacrine cells. Finally, we presented evidence that amacrine cell feedback regulation helps maintain light sensitivity in ambient light. Our findings suggest plausible molecular underpinnings and physiological effects of feedback regulation from amacrine cells to photoreceptors. These results provide new mechanistic insight into how synaptic feedback regulation can participate in network processing by modulating neural information transfer and circuit excitability. |
format | Online Article Text |
id | pubmed-4382183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43821832015-04-09 I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors Hu, Wen Wang, Tingting Wang, Xiao Han, Junhai PLoS Biol Research Article In both vertebrates and invertebrates, photoreceptors’ output is regulated by feedback signals from interneurons that contribute to several important visual functions. Although synaptic feedback regulation of photoreceptors is known to occur in Drosophila, many questions about the underlying molecular mechanisms and physiological implementation remain unclear. Here, we systematically investigated these questions using a broad range of experimental methods. We isolated two I(h) mutant fly lines that exhibit rhythmic photoreceptor depolarization without light stimulation. We discovered that I(h) channels regulate glutamate release from amacrine cells by modulating calcium channel activity. Moreover, we showed that the eye-enriched kainate receptor (EKAR) is expressed in photoreceptors and receives the glutamate signal released from amacrine cells. Finally, we presented evidence that amacrine cell feedback regulation helps maintain light sensitivity in ambient light. Our findings suggest plausible molecular underpinnings and physiological effects of feedback regulation from amacrine cells to photoreceptors. These results provide new mechanistic insight into how synaptic feedback regulation can participate in network processing by modulating neural information transfer and circuit excitability. Public Library of Science 2015-04-01 /pmc/articles/PMC4382183/ /pubmed/25831426 http://dx.doi.org/10.1371/journal.pbio.1002115 Text en © 2015 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Wen Wang, Tingting Wang, Xiao Han, Junhai I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors |
title | I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors |
title_full | I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors |
title_fullStr | I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors |
title_full_unstemmed | I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors |
title_short | I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors |
title_sort | i(h) channels control feedback regulation from amacrine cells to photoreceptors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382183/ https://www.ncbi.nlm.nih.gov/pubmed/25831426 http://dx.doi.org/10.1371/journal.pbio.1002115 |
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