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I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors

In both vertebrates and invertebrates, photoreceptors’ output is regulated by feedback signals from interneurons that contribute to several important visual functions. Although synaptic feedback regulation of photoreceptors is known to occur in Drosophila, many questions about the underlying molecul...

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Detalles Bibliográficos
Autores principales: Hu, Wen, Wang, Tingting, Wang, Xiao, Han, Junhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382183/
https://www.ncbi.nlm.nih.gov/pubmed/25831426
http://dx.doi.org/10.1371/journal.pbio.1002115
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author Hu, Wen
Wang, Tingting
Wang, Xiao
Han, Junhai
author_facet Hu, Wen
Wang, Tingting
Wang, Xiao
Han, Junhai
author_sort Hu, Wen
collection PubMed
description In both vertebrates and invertebrates, photoreceptors’ output is regulated by feedback signals from interneurons that contribute to several important visual functions. Although synaptic feedback regulation of photoreceptors is known to occur in Drosophila, many questions about the underlying molecular mechanisms and physiological implementation remain unclear. Here, we systematically investigated these questions using a broad range of experimental methods. We isolated two I(h) mutant fly lines that exhibit rhythmic photoreceptor depolarization without light stimulation. We discovered that I(h) channels regulate glutamate release from amacrine cells by modulating calcium channel activity. Moreover, we showed that the eye-enriched kainate receptor (EKAR) is expressed in photoreceptors and receives the glutamate signal released from amacrine cells. Finally, we presented evidence that amacrine cell feedback regulation helps maintain light sensitivity in ambient light. Our findings suggest plausible molecular underpinnings and physiological effects of feedback regulation from amacrine cells to photoreceptors. These results provide new mechanistic insight into how synaptic feedback regulation can participate in network processing by modulating neural information transfer and circuit excitability.
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spelling pubmed-43821832015-04-09 I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors Hu, Wen Wang, Tingting Wang, Xiao Han, Junhai PLoS Biol Research Article In both vertebrates and invertebrates, photoreceptors’ output is regulated by feedback signals from interneurons that contribute to several important visual functions. Although synaptic feedback regulation of photoreceptors is known to occur in Drosophila, many questions about the underlying molecular mechanisms and physiological implementation remain unclear. Here, we systematically investigated these questions using a broad range of experimental methods. We isolated two I(h) mutant fly lines that exhibit rhythmic photoreceptor depolarization without light stimulation. We discovered that I(h) channels regulate glutamate release from amacrine cells by modulating calcium channel activity. Moreover, we showed that the eye-enriched kainate receptor (EKAR) is expressed in photoreceptors and receives the glutamate signal released from amacrine cells. Finally, we presented evidence that amacrine cell feedback regulation helps maintain light sensitivity in ambient light. Our findings suggest plausible molecular underpinnings and physiological effects of feedback regulation from amacrine cells to photoreceptors. These results provide new mechanistic insight into how synaptic feedback regulation can participate in network processing by modulating neural information transfer and circuit excitability. Public Library of Science 2015-04-01 /pmc/articles/PMC4382183/ /pubmed/25831426 http://dx.doi.org/10.1371/journal.pbio.1002115 Text en © 2015 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Wen
Wang, Tingting
Wang, Xiao
Han, Junhai
I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
title I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
title_full I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
title_fullStr I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
title_full_unstemmed I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
title_short I(h) Channels Control Feedback Regulation from Amacrine Cells to Photoreceptors
title_sort i(h) channels control feedback regulation from amacrine cells to photoreceptors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382183/
https://www.ncbi.nlm.nih.gov/pubmed/25831426
http://dx.doi.org/10.1371/journal.pbio.1002115
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